Ye Yaqin, Chang Yanting, Zhang Wenbo, Chu Tiankui, Tian Hanchen, Deng Yayun, Jiang Zehui, Ma Yanjun, Hu Tao
International Center for Bamboo and Rattan, Beijing 100102, China.
Key Laboratory of National Forestry and Grassland Administration, on Bamboo & Rattan Science and Technology, Beijing 100102, China.
Plants (Basel). 2025 May 19;14(10):1520. doi: 10.3390/plants14101520.
As climate change intensifies soil drought and salinization, enhancing the drought and salt tolerance of moso bamboo () is urgent. DHN genes are crucial for plant stress responses and have gained attention in plant resistance to drought and salinity. This study identified nine DHN family members (-) from moso bamboo, which were classified into KS-type, YKS-type, and YKS-type dehydrins based on their characteristic motifs. We employed integrated bioinformatics approaches to analyze their gene structure, phylogeny, biological properties, and expression patterns under various stress conditions. Five genes ), which may have significant functional roles in moso bamboo, were selected for cloning. Subcellular localization experiments showed that YKS-type dehydrins (PeDHN2/5/6) localized to both the nucleus and the plasma membrane, while KS-type dehydrins (/8) were exclusively localized to the plasma membrane, indicating functional differentiation. qRT-PCR analysis revealed that the expression of was significantly responsive to stress treatments with ABA, NaCl, and PEG. Additionally, overexpressing these genes in rice significantly enhanced seed germination rates and root development under salt and ABA stress, further confirming that PeDHN2/4/5/6/8 contribute to enhancing plant stress tolerance. Yeast one-hybrid assays demonstrated that two proteins could bind to the promoter of , suggesting that may regulate stress responses through the ABA signaling pathway. Thus, these findings demonstrate that PeDHN2/4/5/6/8 are closely related to the response of moso bamboo to drought and saline-alkali environments. This research offers insights for moso bamboo cultivation and theoretical foundations for bamboo genetic improvement in stress environments.
随着气候变化加剧土壤干旱和盐碱化，提高毛竹的耐旱性和耐盐性迫在眉睫。脱水素（DHN）基因对植物应激反应至关重要，在植物抗旱和抗盐方面受到关注。本研究从毛竹中鉴定出9个DHN家族成员（-），根据其特征基序分为KS型、YKS型和YKS型脱水素。我们采用综合生物信息学方法分析它们的基因结构、系统发育、生物学特性以及在各种胁迫条件下的表达模式。选择了5个可能在毛竹中具有重要功能作用的基因进行克隆。亚细胞定位实验表明，YKS型脱水素（PeDHN2/5/6）定位于细胞核和质膜，而KS型脱水素（/8）仅定位于质膜，表明存在功能分化。qRT-PCR分析显示，的表达对ABA、NaCl和PEG胁迫处理有显著响应。此外，在水稻中过表达这些基因显著提高了盐胁迫和ABA胁迫下的种子萌发率和根系发育，进一步证实PeDHN2/4/5/6/8有助于增强植物的胁迫耐受性。酵母单杂交试验表明，两种蛋白可以与的启动子结合，表明可能通过ABA信号通路调节应激反应。因此，这些发现表明PeDHN2/4/5/6/8与毛竹对干旱和盐碱环境的响应密切相关。本研究为毛竹栽培提供了见解，并为胁迫环境下竹子的遗传改良奠定了理论基础。
