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大豆基因的过表达增强了转基因 的抗旱性。

Overexpression of soybean gene enhances drought resistance of transgenic .

机构信息

College of Agronomy, Jilin Agricultural University, Changchun, China.

College of Life Sciences, Jilin Agricultural University, Changchun, China.

出版信息

GM Crops Food. 2024 Dec 31;15(1):118-129. doi: 10.1080/21645698.2024.2327116. Epub 2024 Apr 2.

Abstract

Soybean is one of the important oil crops and a major source of protein and lipids. Drought can cause severe soybean yields. Dehydrin protein (DHN) is a subfamily of LEA proteins that play an important role in plant responses to abiotic stresses. In this study, the soybean gene was cloned and induced under a variety of abiotic stresses. Results showed that the gene response was more pronounced under drought induction. Subcellular localization results indicated that the protein was localized in the cytoplasm. The role of transgenic Arabidopsis plants in drought stress response was further studied. Under drought stress, the germination rate, root length, chlorophyll, proline, relative water content, and antioxidant enzyme content of transgenic Arabidopsis thaliana transgenic genes were higher than those of wild-type plants, and transgenic plants contained less O, HO and MDA contents. In short, the gene can regulate the homeostasis of ROS and enhance the drought resistance of plants.

摘要

大豆是重要的油料作物之一,也是蛋白质和脂类的主要来源。干旱会导致大豆严重减产。脱水素蛋白(DHN)是 LEA 蛋白的一个亚家族,在植物应对非生物胁迫方面发挥着重要作用。本研究克隆了大豆基因,并在多种非生物胁迫下进行了诱导。结果表明,该基因在干旱诱导下的响应更为明显。亚细胞定位结果表明,该蛋白定位于细胞质中。进一步研究了转基因拟南芥植物在干旱胁迫响应中的作用。在干旱胁迫下,转大豆基因的拟南芥的发芽率、根长、叶绿素、脯氨酸、相对含水量和抗氧化酶含量均高于野生型植物,且转基因植物中 O、HO 和 MDA 的含量较少。总之,基因可以调节 ROS 的内稳态,增强植物的抗旱性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c11/10989702/09e69d015ee2/KGMC_A_2327116_F0001_B.jpg

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