Castro David, Concha Christopher, Jamett Fabiola, Ibáñez Cristian, Hurry Vaughan
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90736 Umeå, Sweden.
Laboratorio de Silvogenómica y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad de La Serena, La Serena 1720236, Chile.
Plants (Basel). 2022 Oct 14;11(20):2717. doi: 10.3390/plants11202717.
and , two woody legumes adapted to the arid regions of Chile, have a declining distribution due to the lack of new seedling establishment. This study investigated the potential of both species to establish in soil collected from four locations in Chile, within and outside the species distribution, and to assess the role of the root-colonizing microbiome in seedling establishment and growth. Seedling survival, height, and water potential were measured to assess establishment success and growth. 16S and ITS2 amplicon sequencing was used to characterize the composition of microbial communities from the different soils and to assess the ability of both species to recruit bacteria and fungi from the different soils. Both species were established on three of the four soils. seedlings showed significantly higher survival in foreign soils and maintained significantly higher water potential in Mediterranean soils. Amplicon sequencing showed that the four soils harbored distinct microbial communities. Root-associated microbial composition indicated that preferentially recruited mycorrhizal fungal partners while recruited abundant bacteria with known salt-protective functions. Our results suggest that a combination of edaphic properties and microbial soil legacy are potential factors mediating the establishment success in different soils.
以及两种适应智利干旱地区的木本豆科植物，由于缺乏新的幼苗定植，其分布范围正在缩小。本研究调查了这两个物种在从智利四个地点采集的土壤中定植的潜力，这些地点位于物种分布范围内和范围外，并评估了根际微生物群落在幼苗定植和生长中的作用。测量了幼苗的存活率、高度和水势，以评估定植成功率和生长情况。使用16S和ITS2扩增子测序来表征不同土壤中微生物群落的组成，并评估这两个物种从不同土壤中招募细菌和真菌的能力。这两个物种都在四种土壤中的三种上定植成功。外来土壤中的幼苗存活率显著更高，在地中海土壤中保持显著更高的水势。扩增子测序表明，这四种土壤含有不同的微生物群落。根系相关微生物组成表明，优先招募菌根真菌伙伴，而招募了大量具有已知盐保护功能的细菌。我们的结果表明，土壤性质和微生物土壤遗留物的组合是介导这两个物种在不同土壤中定植成功的潜在因素。
