Wahongan Irma F, Suoth Elly J, Alhumaid Saad, Albayat Hawra, Aljeldah Mohammed, Al Shammari Basim R, Mashraqi Mutaib M, Alshehri Ahmad A, Sulaiman Tarek, Turkistani Safaa A, Alwashmi Ameen S S, Garout Mohammed, Tallei Trina Ekawati, Rabaan Ali A
Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, North Sulawesi, Indonesia.
Administration of Pharmaceutical Care, Al-Ahsa Health Cluster, Ministry of Health, Al-Ahsa 31982, Saudi Arabia.
Vaccines (Basel). 2022 Oct 17;10(10):1734. doi: 10.3390/vaccines10101734.
Dengue fever (DF) continues to be one of the tropical and subtropical health concerns. Its prevalence tends to increase in some places in these regions. This disease is caused by the dengue virus (DENV), which is transmitted through the mosquitoes and . The treatment of DF to date is only supportive and there is no definitive vaccine to prevent this disease. The non-structural DENV protein, RNA-dependent RNA Polymerase (RdRp), is involved in viral replication. The RdRp-derived peptides can be used in the construction of a universal dengue vaccine. These peptides can be utilized as epitopes to induce immunity. This study was an in silico evaluation of the affinity of the potential epitope for the universal dengue vaccine to dendritic cells and the bonds between the epitope and the dendritic cell receptor. The peptide sequence MGKREKKLGEFGKAKG generated from dengue virus subtype 2 (DENV-2) RdRp was antigenic, did not produce allergies, was non-toxic, and had no homology with the human genome. The potential epitope-based vaccine MGKREKKLGEFGKAKG binds stably to dendritic cell receptors with a binding free energy of -474,4 kcal/mol. This epitope is anticipated to induce an immunological response and has the potential to serve as a universal dengue virus vaccine candidate.
登革热（DF）仍然是热带和亚热带地区关注的健康问题之一。在这些地区的一些地方，其发病率呈上升趋势。这种疾病由登革病毒（DENV）引起，通过蚊子传播。迄今为止，登革热的治疗仅为支持性治疗，尚无预防该疾病的特效疫苗。登革病毒的非结构蛋白，即RNA依赖性RNA聚合酶（RdRp），参与病毒复制。源自RdRp的肽可用于构建通用登革热疫苗。这些肽可作为表位诱导免疫。本研究是对通用登革热疫苗潜在表位与树突状细胞的亲和力以及表位与树突状细胞受体之间结合的计算机模拟评估。从登革病毒2型（DENV-2）RdRp产生的肽序列MGKREKKLGEFGKAKG具有抗原性，不会产生过敏反应，无毒，且与人类基因组无同源性。基于潜在表位的疫苗MGKREKKLGEFGKAKG以-474.4千卡/摩尔的结合自由能稳定地与树突状细胞受体结合。预计该表位可诱导免疫反应，有潜力作为通用登革热病毒疫苗候选物。
