L'Oreal Research and Innovation, Clark, NJ, USA.
Skincare Physicians, Chestnut Hill, MA, USA.
Sci Rep. 2022 Oct 27;12(1):18115. doi: 10.1038/s41598-022-22481-w.
Microneedling is a popular skin resurfacing and rejuvenation procedure. In order to develop better adjunct products for consumers, there is a scientific need to establish greater understanding of the mechanism in which microneedling stimulates regeneration within skin. The purpose of this study is to develop a physiologically relevant ex vivo tissue model which closely mimics the actual microneedling procedure to elucidate its mechanism of action. In this study, human ex vivo skin was subjected to microneedling treatment and cultured for 6 days. Histological analysis demonstrated that the ex vivo skin was able to heal from microneedling injury throughout the culture period. Microneedling treatment stimulated proliferation and barrier renewal of the skin. The procedure also increased the levels of inflammatory cytokines and angiogenic growth factors in a dynamic and time dependent fashion. The tissue demonstrated hallmark signs of epidermal regeneration through morphological and molecular changes after the treatment. This is one of the first works to date that utilizes microneedled ex vivo skin to demonstrate its regenerative behavior. Our model recapitulates the main features of the microneedling treatment and enables the evaluation of future cosmetic active ingredients used in conjunction with microneedling.
微针是一种流行的皮肤表面重塑和年轻化程序。为了为消费者开发更好的辅助产品，科学界需要更好地了解微针刺激皮肤再生的机制。本研究旨在开发一种生理相关的离体组织模型，该模型可模拟实际的微针处理过程，以阐明其作用机制。在这项研究中，人体离体皮肤进行微针处理，并培养 6 天。组织学分析表明，离体皮肤在整个培养期间能够从微针损伤中愈合。微针处理刺激了皮肤的增殖和屏障更新。该过程还以动态和时间依赖的方式增加了炎症细胞因子和血管生成生长因子的水平。该组织在治疗后通过形态和分子变化表现出表皮再生的标志性特征。这是迄今为止首次利用微针处理的离体皮肤来证明其再生行为的工作之一。我们的模型再现了微针处理的主要特征，并能够评估与微针处理联合使用的未来化妆品活性成分。
