Raquel Ferreira Paulo Iolanda, Basílio de Oliveira Caland Ricardo, Orlando Muñoz Cadavid Cesar, Martins Melo Giovanna, Soares De Castro Bezerra Liliane, Pons Elsa, Peña Leandro, de Paula Oliveira Riva
Departamento de Biologia Celular e Genética, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Instituto Federal de Educação, Ciência e Tecnologia do Piauí-IFPI, Brazil.
Food Chem (Oxf). 2022 Oct 17;5:100141. doi: 10.1016/j.fochms.2022.100141. eCollection 2022 Dec 30.
orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared to its conventional counterpart (CV). Using , we found that animals treated with GS showed a greater reduction in intracellular reactive oxygen species (ROS) which is associated with a greater resistance to oxidative stress and induction of the expression of antioxidant genes. Moreover, animals treated with GS orange showed a more effective protection against β-amyloid proteotoxicity and greater hypolipidemic effect under high glucose diet compared to animals treated with CV. These data demonstrate that the increased amount of β-carotene in orange actually provides a greater beneficial effect in and a valuable proof of principle to support further studies in mammals and humans.
橙汁是β-胡萝卜素的优质膳食来源，β-胡萝卜素是一种著名的抗氧化剂。然而，大多数品种的β-胡萝卜素浓度相对较低。我们通过代谢工程策略（GS）培育出一种新的橙子，其β-胡萝卜素含量比传统对应品种（CV）增加了33.72倍。通过研究，我们发现用GS处理的动物细胞内活性氧（ROS）的减少幅度更大，这与对氧化应激的更强抵抗力以及抗氧化基因表达的诱导有关。此外，与用CV处理的动物相比，用GS橙子处理的动物在高糖饮食下对β-淀粉样蛋白毒性具有更有效的保护作用，且降血脂效果更佳。这些数据表明，橙子中β-胡萝卜素含量的增加实际上在[具体方面]提供了更大的有益效果，并且为支持在哺乳动物和人类中的进一步研究提供了有价值的原理证明。
