Clinical Laboratory, DongYang People's Hospital, 60 West Wuning Road, Dongyang, 322100, Zhejiang, China.
Reproductive Medicine Center, DongYang People's Hospital, 60 West Wuning Road, Dongyang, 322100, Zhejiang, China.
Sci Rep. 2022 Oct 28;12(1):18166. doi: 10.1038/s41598-022-23190-0.
Adaptive immune cells prevent solid tumor progression by targeting and killing tumor cells. However, there are no comprehensive studies on peripheral circulating adaptive immune cell characterization in colorectal cancer (CRC) patients or the effect of tumor-node-metastasis (TNM) stages on these cells. In this study, the number, phenotype, and function of different subsets of circulating adaptive immune cells in peripheral blood of CRC patients were analyzed. We found remarkable differences in CRC patients compared with those in healthy controls, including reduced absolute counts of total T cells, helper T lymphocytes (Th), cytotoxic T lymphocytes (Tc), and double-negative T lymphocytes, a decreased proportion of INF-γ cells in total T cells and Th, and increased percentages of B cells, plasmablasts, and activated T cells. Compared with early-stage CRC patients, advanced-stage CRC patients showed more severe immunosenescence, which manifested as decreased proportions of CD8 naive T cells with strong proliferative ability and CD8 central memory T cells with immune surveillance function. Proportions and absolute counts of CD8 and CD4 terminally differentiated effector memory T cells were increased, indicating immunosenescence. The immune cell characteristics analyzed in this study serve as a starting point for further research to determine potential clinical implications.
适应性免疫细胞通过靶向和杀伤肿瘤细胞来预防实体瘤的进展。然而，目前还没有关于结直肠癌（CRC）患者外周循环适应性免疫细胞特征或肿瘤-淋巴结-转移（TNM）分期对这些细胞影响的综合研究。在这项研究中，分析了 CRC 患者外周血中不同循环适应性免疫细胞亚群的数量、表型和功能。我们发现 CRC 患者与健康对照组相比存在显著差异，包括总 T 细胞、辅助性 T 淋巴细胞（Th）、细胞毒性 T 淋巴细胞（Tc）和双阴性 T 淋巴细胞的绝对计数减少，总 T 细胞和 Th 中 IFN-γ细胞的比例降低，B 细胞、浆母细胞和活化 T 细胞的百分比增加。与早期 CRC 患者相比，晚期 CRC 患者表现出更严重的免疫衰老，这表现为具有强大增殖能力的 CD8 幼稚 T 细胞和具有免疫监视功能的 CD8 中央记忆 T 细胞的比例降低。CD8 和 CD4 终末分化效应记忆 T 细胞的比例和绝对计数增加，表明存在免疫衰老。本研究分析的免疫细胞特征为进一步研究确定潜在的临床意义提供了起点。
