Department of Chemical Pathology, Medical Research Institute, Alexandria University, Egypt.
Department of Pharmacology and Therapeutics, Faculty of Pharmacy, Pharos University, Egypt.
Asian Pac J Cancer Prev. 2022 Oct 1;23(10):3361-3370. doi: 10.31557/APJCP.2022.23.10.3361.
evaluating the role of FOXO3 mRNA and mi RNA 182-5P expression levels in BC patients.
25 Samples of breast cancer and paired samples of non-cancerous tissues from the same resected breast were obtained from 25 female patients suffering from breast cancer and examined and analyzed by real time PCR to detect the expression levels of FOXO3 mRNA and mi RNA 182-5P. Patients' data were collected from patients medical records.
Foxo3 m RNA expression was down regulated in BC tissues (1.37± 1.96) as compared to control group (23.62 ± 54.39) and decreased FOXO3 expression was associated with larger tumor size (p= 0.046), late histopathological grading (p= 0.002), late TNM staging (<0.001) and increased miR-182 expression (p= 0.025). We found that expression level of miR-182 was significantly higher among breast cancer group (1.10±1.15) as compared to the control group (0.58±0.96 ) with p value = 0.017. We noted a significant increased expression associated with larger tumor size (p= 0.002), late histopathological grading (p= 0.008), late TNM staging (p= 0.002) and decreased FOXO3 expression (p= 0.025). A significant negative correlation between miR-182 and FOXO3 mRNA fold expression with r = - 0.447, and a p value of 0.025, this could be attributed to miRNA targeting FOXO gene.
Down regulation of FOXO3 and up regulation of miR-182 expression was associated with advanced breast cancer. The negative correlation between miR-182 and FOXO3 mRNA could be attributed to miRNA targeting FOXO gene.
评估 FOXO3mRNA 和 miR-182-5P 表达水平在乳腺癌患者中的作用。
从 25 名女性乳腺癌患者的同一切除乳房中获得 25 例乳腺癌样本和配对的非癌组织样本，并通过实时 PCR 检测 FOXO3mRNA 和 miR-182-5P 的表达水平进行检查和分析。患者数据从患者的病历中收集。
与对照组（23.62 ± 54.39）相比，BC 组织中的 Foxo3m RNA 表达下调（1.37 ± 1.96），并且降低的 FOXO3 表达与更大的肿瘤大小（p = 0.046），晚期组织病理学分级（p = 0.002），晚期 TNM 分期（<0.001）和 miR-182 表达增加（p = 0.025）相关。我们发现，与对照组（0.58 ± 0.96）相比，乳腺癌组的 miR-182 表达水平显着升高（1.10 ±1.15），p 值= 0.017。我们注意到与较大的肿瘤大小（p = 0.002），晚期组织病理学分级（p = 0.008），晚期 TNM 分期（p = 0.002）和 FOXO3 表达降低（p = 0.025）相关的显着增加表达。miR-182 和 FOXO3mRNA 折叠表达之间存在显着的负相关，r = -0.447，p 值为 0.025，这可能归因于 miRNA 靶向 FOXO 基因。
FOXO3 的下调和 miR-182 表达的上调与晚期乳腺癌有关。miR-182 和 FOXO3mRNA 之间的负相关可能归因于 miRNA 靶向 FOXO 基因。
