School of Public Health, University of Nevada Reno, Reno, NV, USA.
Department of Psychology Institute for Neuroscience, University of Nevada Reno, Reno, NV, USA.
Methods Mol Biol. 2023;2426:361-374. doi: 10.1007/978-1-0716-1967-4_16.
MetaMSD is a proteomic software that integrates multiple quantitative mass spectrometry data analysis results using statistical summary combination approaches. By utilizing this software, scientists can combine results from their pilot and main studies to maximize their biomarker discovery while effectively controlling false discovery rates. It also works for combining proteomic datasets generated by different labeling techniques and/or different types of mass spectrometry instruments. With these advantages, MetaMSD enables biological researchers to explore various proteomic datasets in public repositories to discover new biomarkers and generate interesting hypotheses for future studies. In this protocol, we provide a step-by-step procedure on how to install and perform a meta-analysis for quantitative proteomics using MetaMSD.
MetaMSD 是一种蛋白质组学软件，它使用统计汇总组合方法整合多个定量质谱数据分析结果。通过使用该软件，科学家可以将来自其先导和主要研究的结果进行组合，以最大化他们的生物标志物发现，同时有效地控制假发现率。它还可用于组合由不同标记技术和/或不同类型的质谱仪器生成的蛋白质组数据集。有了这些优势，MetaMSD 使生物研究人员能够探索公共存储库中的各种蛋白质组数据集，以发现新的生物标志物，并为未来的研究生成有趣的假设。在本方案中，我们提供了使用 MetaMSD 进行定量蛋白质组学元分析的逐步安装和执行过程。
