Kareem Babajide, Irondi Emmanuel Anyachukwu, Alamu Emmanuel Oladeji, Ajani Emmanuel Oladipo, Abass Adebayo, Adesokan Michael, Parkes Elizabeth, Maziya-Dixon Busie
Department of Medical Biochemistry and Pharmacology, Kwara State University, Ilorin, Nigeria.
Food and Nutrition Sciences Laboratory, International Institute of Tropical Agriculture, Lusaka, Zambia.
Front Nutr. 2022 Oct 14;9:894843. doi: 10.3389/fnut.2022.894843. eCollection 2022.
Yellow-fleshed cassava root (YFCR) is processed into traditional products that may influence its bioactivities. In this study, the antioxidant and anti-hyperglycaemic activities of three traditional products ( and ) from five genotypes (IITA-TMS-IBA070337, 182961, 182962, 182986, 183044) of YFCR were evaluated. The YFCR genotypes were grown at the International Institute of Tropical Agriculture (IITA) research field, Ibadan. The bioactive constituents (total carotenoids, total phenolics, tannins and total flavonoids), antioxidant [2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) radical cation (ABTS) and 1,1-diphenyl-2- picrylhydrazyl radical (DPPH) scavenging capacities, and reducing power], and starch-digesting enzymes (α-amylase and α-glucosidase) inhibitory activities of the products were determined using standard laboratory methods. The glucose response of the products was assessed in human subjects. The concentrations of the bioactive constituents of the products from different genotypes varied significantly ( < 0.05). The ABTS and DPPH scavenging capacities and the reducing power of the products also differed significantly ( < 0.05), such that the from IITA-TMS-IBA182962, IITA-TMS-IBA070337 and IITA-TMS-IBA070337 had the strongest ABTS and DPPH scavenging capacities, and reducing power, respectively. The α-amylase and α-glucosidase inhibitory activities of the three products differed significantly ( < 0.05), with the from IITA-TMS-IBA070337 and IITA-TMS-IBA07033 having the strongest α-amylase and α-glucosidase inhibitory activity, respectively. Also, the from IITA-TMS-182986 had the least glucose response, while the from IITA-TMS-IBA070337 had the highest glucose response. Overall, the from different genotypes of YFCR had the most potent antioxidant and starch-digesting enzymes inhibitory activities and the least glucose responses. Hence, may be a promising dietary intervention targeting oxidative stress, hyperglycaemia, and their resultant type 2 diabetes.
黄肉木薯根（YFCR）被加工成可能会影响其生物活性的传统产品。在本研究中，对来自五种基因型（IITA-TMS-IBA070337、182961、182962、182986、183044）的YFCR的三种传统产品（和）的抗氧化和抗高血糖活性进行了评估。YFCR基因型在伊巴丹国际热带农业研究所（IITA）的研究田种植。使用标准实验室方法测定了产品的生物活性成分（总类胡萝卜素、总酚类、单宁和总黄酮）、抗氧化剂[2,2-偶氮二（3-乙基苯并噻唑啉-6-磺酸）自由基阳离子（ABTS）和1,1-二苯基-2-苦基肼自由基（DPPH）清除能力以及还原能力]以及淀粉消化酶（α-淀粉酶和α-葡萄糖苷酶）抑制活性。在人体受试者中评估了产品的葡萄糖反应。不同基因型产品的生物活性成分浓度差异显著（<0.05）。产品的ABTS和DPPH清除能力以及还原能力也有显著差异（<0.05），以至于来自IITA-TMS-IBA182962、IITA-TMS-IBA070337和IITA-TMS-IBA070337的产品分别具有最强的ABTS和DPPH清除能力以及还原能力。三种产品的α-淀粉酶和α-葡萄糖苷酶抑制活性差异显著（<0.05），来自IITA-TMS-IBA070337和IITA-TMS-IBA07033的产品分别具有最强的α-淀粉酶和α-葡萄糖苷酶抑制活性。此外，来自IITA-TMS-182986的产品葡萄糖反应最小，而来自IITA-TMS-IBA070337的产品葡萄糖反应最大。总体而言，不同基因型的YFCR产品具有最有效的抗氧化和淀粉消化酶抑制活性以及最小的葡萄糖反应。因此，可能是针对氧化应激、高血糖及其导致的2型糖尿病的一种有前景的饮食干预措施。
