Cheng Wenhui, Zhang Man, Cheng Tangren, Wang Jia, Zhang Qixiang
Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, China.
Front Genet. 2022 Oct 12;13:1013822. doi: 10.3389/fgene.2022.1013822. eCollection 2022.
(Aux/IAAs), an early auxin-responsive gene family, is important for plant growth and development. To fully comprehend the character of genes in woody plants, we identified 19 genes in and dissected their protein domains, phylogenetic relationship, gene structure, promoter, and expression patterns during floral bud flushing, auxin response, and abiotic stress response. The study showed that PmIAA proteins shared conserved Aux/IAA domain, but differed in protein motif composition. 19 genes were divided into six groups (Groups Ⅰ to Ⅵ) based on phylogenetic analysis. The gene duplication analysis showed that segmental and dispersed duplication greatly influenced the expansion of genes. Moreover, we identified and classified the cis-elements of gene promoters and detected elements that are related to phytohormone responses and abiotic stress responses. With expression pattern analysis, we observed the auxin-responsive expression of and in flower bud, stem, and leaf tissues. were possibly involved in abiotic stress responses in . In general, these results laid the theoretical foundation for elaborating the functions of genes in perennial woody plant development.
生长素/吲哚乙酸(Aux/IAAs)是一个早期生长素响应基因家族,对植物生长发育至关重要。为了全面了解木本植物中该基因家族的特征,我们在[植物名称]中鉴定出19个[基因名称]基因,并剖析了它们的蛋白质结构域、系统发育关系、基因结构、启动子以及在花芽萌动、生长素响应和非生物胁迫响应过程中的表达模式。研究表明,梨(Pyrus)IAA蛋白具有保守的Aux/IAA结构域,但在蛋白质基序组成上存在差异。基于系统发育分析,19个[基因名称]基因被分为六组(Ⅰ组至Ⅵ组)。基因复制分析表明,片段重复和分散重复对[基因名称]基因的扩增有很大影响。此外,我们鉴定并分类了[基因名称]基因启动子的顺式元件,并检测到与植物激素响应和非生物胁迫响应相关的元件。通过表达模式分析,我们观察到[基因名称]基因在花芽、茎和叶组织中的生长素响应表达。[基因名称]基因可能参与了[植物名称]的非生物胁迫响应。总体而言,这些结果为阐明[基因名称]基因在多年生木本植物发育中的功能奠定了理论基础。