Lian Conglong, Lan Jinxu, Ma Rui, Li Jingjing, Zhang Fei, Zhang Bao, Liu Xiuyu, Chen Suiqing
School of Pharmacy, Henan University of Chinese Medicine, 156 East Jin-shui Rd., Zhengzhou 450046, China.
Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 East Jin-shui Rd., Zhengzhou 450046, China.
Plants (Basel). 2024 Feb 20;13(5):564. doi: 10.3390/plants13050564.
is a traditional herbal medicine plant, and its folium artemisia argyi is widely in demand due to moxibustion applications globally. The Auxin/indole-3-acetic acid (Aux/IAA, or IAA) gene family has critical roles in the primary auxin-response process, with extensive involvement in plant development and stresses, controlling various essential traits of plants. However, the systematic investigation of the Aux/IAA gene family in remains limited. In this study, a total of 61 Aux/IAA genes were comprehensively identified and characterized. Gene structural analysis indicated that 46 Aux/IAA proteins contain the four typical domains, and 15 Aux/IAA proteins belong to non-canonical IAA proteins. Collinear prediction and phylogenetic relationship analyses suggested that Aux/IAA proteins were grouped into 13 distinct categories, and most Aux/IAA genes might experience gene loss during the tandem duplication process. Promoter -element investigation indicated that Aux/IAA promoters contain a variety of plant hormone response and stress response -elements. Protein interaction prediction analysis demonstrated that AaIAA26/29/7/34 proteins are possibly core members of the Aux/IAA family interaction. Expression analysis in roots and leaves via RNA-seq data indicated that the expression of some AaIAAs exhibited tissue-specific expression patterns, and some AaIAAs were involved in the regulation of salt and saline-alkali stresses. In addition, RT-qPCR results indicated that AaIAA genes have differential responses to auxin, with complex response patterns in response to other hormones, indicating that Aux/IAA may play a role in connecting auxin and other hormone signaling pathways. Overall, these findings shed more light on AaIAA genes and offer critical foundational knowledge toward the elucidation of their function during plant growth, stress response, and hormone networking of Aux/IAA family genes in .
是一种传统草药植物，其艾叶因在全球艾灸应用中需求广泛。生长素/吲哚-3-乙酸（Aux/IAA，或IAA）基因家族在生长素初级响应过程中起关键作用，广泛参与植物发育和胁迫，控制植物的各种重要性状。然而，对[植物名称未给出]中Aux/IAA基因家族的系统研究仍然有限。在本研究中，共全面鉴定和表征了61个Aux/IAA基因。基因结构分析表明，46个Aux/IAA蛋白含有四个典型结构域，15个Aux/IAA蛋白属于非典型IAA蛋白。共线性预测和系统发育关系分析表明，Aux/IAA蛋白被分为13个不同类别，大多数Aux/IAA基因可能在串联重复过程中经历基因丢失。启动子元件研究表明，Aux/IAA启动子包含多种植物激素响应和胁迫响应元件。蛋白质相互作用预测分析表明，AaIAA26/29/7/34蛋白可能是Aux/IAA家族相互作用的核心成员。通过RNA-seq数据对根和叶进行的表达分析表明，一些AaIAAs的表达表现出组织特异性表达模式，一些AaIAAs参与了盐和盐碱胁迫的调控。此外，RT-qPCR结果表明，AaIAA基因对生长素具有不同的响应，对其他激素具有复杂的响应模式，表明Aux/IAA可能在连接生长素和其他激素信号通路中发挥作用。总体而言，这些发现为AaIAAs基因提供了更多信息，并为阐明其在植物生长、胁迫响应以及Aux/IAA家族基因在[植物名称未给出]中的激素网络中的功能提供了关键的基础知识。
