Liu Shixuan, Liu Yanbin, Zhang Xi, Song Xuanlin, Zhang Boxiang, Zhang Yong
Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Health Science Center, Xi'an Jiaotong University, Xi'an, China.
Front Genet. 2022 Oct 13;13:1039440. doi: 10.3389/fgene.2022.1039440. eCollection 2022.
Recent studies have demonstrated the significance of the () gene, which is involved in pathways concerning the modification of RNA structures. functions as a coregulator of cellular transcription and splicing, and participates in the processing of small noncoding RNAs. The aberrant regulation of expression possibly plays a significant role in the genesis of cancer. However, there are no comprehensive pan-cancer studies on . This study is the first to conduct a pan-cancer analysis of for aiding the diagnosis and treatment of cancer. The gene expression, genetic alterations, protein phosphorylation, promoter methylation, immune infiltration, and enrichment analyses of were performed using data retrieved from The Cancer Genome Atlas (TCGA), Genotype-tissue Expression (GTEx), Human Protein Atlas (HPA), Tumor Immunological Estimation Resource 2.0 (TIMER2.0), Gene Expression Profiling Interactive Analysis (GEPIA), DNA methylation interactive visualization database (DNMIVD), and Search Tool for the Retrieval of Interaction Genes/Proteins (STRING). Data analyses were performed with the R software and other webtools. The expression of DDX5 mRNA decreased significantly in 17 cancer types, but increased significantly in eight cancer types. The enhanced expression of DDX5 mRNA in the tumor samples was related to decreased overall survival (OS), progression-free interval (PFI), and disease-specific survival (DSS) in three cancers, but increased OS, PFI, and DSS in other cancers. The DNA promoter methylation level was significantly reduced in eight cancer types, and there were exceptions in the methylation levels of the promoter in four cancer types. The expression of DDX5 mRNA was highly correlated with the infiltration of CD8 T cells, cancer-associated fibroblasts, and B cells in a wide variety of malignancies. The findings revealed a strong association between and its co-expressed genes in numerous cancer types. Enrichment analysis suggested that was associated with multiple cellular pathways, including RNA splicing, Notch signaling pathway, and viral carcinogenesis, which was consistent with the results of previous studies. The findings obtained herein provide further information on the oncogenic potential of in diverse tumor types. We propose that has important roles in tumor immunity and the diagnosis of cancer.
最近的研究已经证明了（）基因的重要性，该基因参与了与RNA结构修饰相关的途径。它作为细胞转录和剪接的共调节因子发挥作用，并参与小非编码RNA的加工过程。该基因表达的异常调控可能在癌症的发生中起重要作用。然而，目前尚无关于该基因的全面泛癌研究。本研究首次对该基因进行泛癌分析，以辅助癌症的诊断和治疗。利用从癌症基因组图谱（TCGA）、基因型-组织表达（GTEx）、人类蛋白质图谱（HPA）、肿瘤免疫评估资源2.0（TIMER2.0）、基因表达谱交互式分析（GEPIA）、DNA甲基化交互式可视化数据库（DNMIVD）以及检索相互作用基因/蛋白质的搜索工具（STRING）中获取的数据，对该基因的表达、基因改变、蛋白质磷酸化、启动子甲基化、免疫浸润和富集分析进行了研究。数据分析使用R软件和其他网络工具进行。在17种癌症类型中，DDX5 mRNA的表达显著降低，但在8种癌症类型中显著升高。肿瘤样本中DDX5 mRNA表达的增强与三种癌症中总生存期（OS）、无进展生存期（PFI）和疾病特异性生存期（DSS）的降低相关，但在其他癌症中OS、PFI和DSS升高。在8种癌症类型中，DNA启动子甲基化水平显著降低，在4种癌症类型中该基因启动子的甲基化水平存在例外情况。在多种恶性肿瘤中，DDX5 mRNA的表达与CD8 T细胞、癌症相关成纤维细胞和B细胞的浸润高度相关。研究结果揭示了该基因与其在多种癌症类型中共表达基因之间的紧密关联。富集分析表明，该基因与多种细胞途径相关，包括RNA剪接、Notch信号通路和病毒致癌作用，这与先前的研究结果一致。本文获得的研究结果提供了关于该基因在不同肿瘤类型中致癌潜力的进一步信息。我们认为该基因在肿瘤免疫和癌症诊断中具有重要作用。
