Shen Xuezhen, Qu Feng, Pei Yilun, Lei Simeng, Xia Suhang, Liang Jing, Li Shan, Sun Xun, Liu Liang
Department of Orthopedics, Beijing Luhe Hospital, Affiliated to Capital Medical University, Beijing, China.
Foot and Ankle Surgery Center, Beijing Tongren Hospital, Affiliated to Capital Medical University, Beijing, China.
Front Neurol. 2022 Oct 13;13:867711. doi: 10.3389/fneur.2022.867711. eCollection 2022.
An increasing number of nerve guide scaffolds have been used to replace the "gold-standard" autologous nerve graft for repairing peripheral nerve defects, but nerve regeneration is usually far from complete.
Here, we designed and prepared two functionalized self-assembling peptides (SAP) with the IKVAV and KLT sequences, which were derived from the combination of laminin and VEGF, respectively. Their mixtures were also obtained to combine the effects of neuroprotective and neurotrophic and proangiogenic factors.
The beneficial effect of peptide gels on nerve regeneration was evaluated using Schwann cells (SCs). As a useful intraluminal filling, a three-dimensional (3D) functionalized self-assembling peptide (SAP) nanofiber hydrogel was formed in the hollow lumen of chitosan conduits under physiological conditions. , the combination of the two functionalized SAP gels containing a chitosan nerve conduit significantly accelerated nerve healing and enhanced morphological repair.
Based on the current findings, the combined application of two functionalized SAP gels with chitosan nerve conduit is a promising therapy for the engineering of peripheral nerve regeneration.
越来越多的神经引导支架已被用于替代“金标准”自体神经移植来修复周围神经缺损，但神经再生通常远未完全实现。
在此，我们设计并制备了两种分别具有IKVAV和KLT序列的功能化自组装肽（SAP），它们分别源自层粘连蛋白和血管内皮生长因子（VEGF）的组合。还获得了它们的混合物，以结合神经保护、神经营养和促血管生成因子的作用。
使用雪旺细胞（SCs）评估了肽凝胶对神经再生的有益作用。作为一种有用的腔内填充物，在生理条件下，壳聚糖导管的中空管腔内形成了三维（3D）功能化自组装肽（SAP）纳米纤维水凝胶。含有壳聚糖神经导管的两种功能化SAP凝胶的组合显著加速了神经愈合并增强了形态修复。
基于目前的研究结果，两种功能化SAP凝胶与壳聚糖神经导管的联合应用是一种有前景的周围神经再生工程治疗方法。
