Zinc ion detection using a benzothiazole-based highly selective fluorescence "turn-on" chemosensor and its real-time application.

A new photochromic fluorescence chemosensor was devised and effectively synthesized using benzothiazole and imidazopyridine derivatives. A "turn-on" fluorescence sensor BIPP for Zn detection was developed and has a quick response, excellent sensitivity, and remarkable selectivity over other metal ions. When Zn was added to the BIPP solution, a new strong fluorescence emission peak at 542 nm formed with a considerable increase in intensity. The fluorescence color of the BIPP solution changed from blue to bright green. The binding ratio 8 : 2 was found between BIPP and Zn by the results of Job's plot, HRMS and H-NMR. The detection limit (LOD) of BIPP towards Zn was determined to be 2.36 × 10, which is remarkably low. The ability to detect Zn in real water samples demonstrates that BIPP may also be used in environmental systems. Additionally, BIPP can be used to measure Zn levels in living cells.