Tavarideh Farhad, Pourahmad Fazel, Nemati Mostafa
Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran.
Vet Res Forum. 2022 Sep;13(3):409-415. doi: 10.30466/vrf.2021.529714.3174. Epub 2022 Sep 15.
To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of . Using sequence analysis targeting 16S rRNA gene, eight genera, including , , , , , , and were identified. Antibacterial activity of endophytic bacteria was examined against some test bacteria, employing agar well diffusion method. Out of 31 endophytic bacterial isolates, 24(77.42%) isolates showed significant antimicrobial activity against , 17(54.84%) isolates exhibited maximum activity against , 14(45.16%) isolates against and 5(16.13%) isolates showed positive activity against .The results obtained in this study suggested that the medicinal plant, is is a potent source of endophytic bacteria with antibacterial activity and offers promise for discovery of more impressive biological compounds.
为了探究内生细菌的多样性并评估其抗菌活性，本研究选取了健康的药用植物。从该植物表面消毒后的组织（根、茎和叶）中分离出100株内生细菌。通过针对16S rRNA基因的序列分析，鉴定出了8个属，包括[具体属名1]、[具体属名2]、[具体属名3]、[具体属名4]、[具体属名5]、[具体属名6]、[具体属名7]和[具体属名8]。采用琼脂扩散法检测内生细菌对一些测试细菌的抗菌活性。在31株内生细菌分离株中，24株（77.42%）对[测试细菌1]表现出显著的抗菌活性，17株（54.84%）对[测试细菌2]表现出最大活性，14株（45.16%）对[测试细菌3]有活性，5株（16.13%）对[测试细菌4]表现出阳性活性。本研究获得的结果表明，该药用植物是具有抗菌活性的内生细菌的重要来源，有望发现更具吸引力的生物化合物。
