Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, China.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, Guizhou, China.
Curr Microbiol. 2022 Nov 3;79(12):377. doi: 10.1007/s00284-022-03083-1.
Hospital grassplot soil is an important repository of pathogenic fungi exposed to the hospital environment, and the diffusion of these fungi-containing soil particles in the air increases the risk of nosocomial fungal infections. In this study, from the perspective of soil microbes-plant holobiont, four medicinal plants Mirabilis jalapa, Artemisia argyi, Viola philippica, and Plantago depressa were used as materials, based on ITS high-throughput amplicon sequencing and simulated pot experiments to explore the effect of medicinal plants on the fungal community in hospital grassplot soil, in order to provide a new exploration for hospital grassplot soil remediation. The results showed that the fungal community ecological guilds in primary test soil was mainly pathogen, and the abundance of animal pathogen with potential threats to human reached 61.36%. After planting medicinal plants, the composition and function of soil fungal community changed significantly. Although this change varied with plant species and growth stages, all samples collected in the pot experiment showed that the pathogen abundance decreased and the saprotroph abundance increased. In addition, 45 of the 46 core fungal genera defined in all potted samples were present in primary test soil, and many of them were human potential pathogens. These findings imply that the idea of enhancing soil quality in hospital grassplot soil by planting specific plants is feasible. However, the initial fungal community of the hospital grassplot soil has a certain stability, and it is difficult to completely eliminate the threat of pathogenic fungi by planting medicinal plants.
医院草坪土壤是暴露于医院环境中的病原真菌的重要储存库，这些含真菌土壤颗粒在空气中的扩散增加了医院真菌感染的风险。在这项研究中，从土壤微生物-植物整体的角度出发，我们选择了四种药用植物：苘麻、艾蒿、堇菜和车前草，作为材料，基于 ITS 高通量扩增子测序和模拟盆栽实验，探索了药用植物对医院草坪土壤中真菌群落的影响，以期为医院草坪土壤修复提供新的探索思路。结果表明，初步测试土壤中的真菌群落生态类群主要是病原体，对人类具有潜在威胁的动物病原体的丰度达到了 61.36%。种植药用植物后，土壤真菌群落的组成和功能发生了显著变化。尽管这种变化因植物种类和生长阶段而异，但盆栽实验中采集的所有样本均表明，病原体的丰度降低，腐生菌的丰度增加。此外，在所有盆栽样本中定义的 46 个核心真菌属中有 45 个都存在于初步测试土壤中，其中许多是人类潜在的病原体。这些发现表明，通过种植特定植物来提高医院草坪土壤质量的想法是可行的。然而，医院草坪土壤的初始真菌群落具有一定的稳定性，通过种植药用植物很难完全消除病原真菌的威胁。
