Chen Chaoqun, Chen Hongxu, Chen Yuanfei, Yang Wenlong, Li Mengyao, Sun Bo, Song Haiyan, Tang Wenjing, Zhang Yao, Gong Ronggao
College of Horticulture, Sichuan Agricultural University, Chengdu, China.
Front Plant Sci. 2022 Oct 18;13:1041068. doi: 10.3389/fpls.2022.1041068. eCollection 2022.
Gibberellin (GA) is an important phytohormone that can participate in various developmental processes of plants. The study found that application of GA can induce parthenocarpy fruit and improve fruit set. However, the use of GA affects endogenous hormones in fruits, thereby affecting fruit quality. This study mainly investigates the effect of exogenous GA on endogenous hormones in sweet cherries. The anabolic pathways of each hormone were analyzed by metabolome and transcriptome to identify key metabolites and genes that affect endogenous hormones in response to exogenous GA application. Results showed that exogenous GA led to a significant increase in the content of abscisic acid (ABA) and GA and affected jasmonic acid (JA) and auxin (IAA). At the same time, the key structural genes affecting the synthesis of various hormones were preliminarily determined. Combined with transcription factor family analysis, genes were found to be more sensitive to the use of exogenous GA, especially the genes belonging to Group III (, , , , and ). These transcription factors can combine with the promoters of , , and other genes to regulate the content of endogenous hormones. These findings lay the foundation for the preliminary determination of the mechanism of GA's effect on endogenous hormones in sweet cherry and the biological function of transcription factors.
赤霉素（GA）是一种重要的植物激素，可参与植物的各种发育过程。研究发现，施用GA可诱导单性结实果实并提高坐果率。然而，GA的使用会影响果实中的内源激素，从而影响果实品质。本研究主要探讨外源GA对甜樱桃内源激素的影响。通过代谢组学和转录组学分析各激素的合成途径，以鉴定响应外源GA施用影响内源激素的关键代谢物和基因。结果表明，外源GA导致脱落酸（ABA）和GA含量显著增加，并影响茉莉酸（JA）和生长素（IAA）。同时，初步确定了影响各种激素合成的关键结构基因。结合转录因子家族分析，发现某些基因对外源GA的使用更为敏感，尤其是属于第三组（、、、和）的基因。这些转录因子可与、等基因的启动子结合，调节内源激素的含量。这些发现为初步确定GA对甜樱桃内源激素的作用机制及转录因子的生物学功能奠定了基础。
