Department of Bioresource Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga, Shizuoka 422-8529, Japan.
Cells. 2021 Feb 3;10(2):308. doi: 10.3390/cells10020308.
Combined spraying of gibberellin (GA) and prohydrojasmon (PDJ) was an effective method to reduce peel puffing in Satsuma mandarins. However, in the GA-and-PDJ combined treatment, fruit color development was delayed during the ripening process. In the present study, to improve the coloration of the GA and PDJ-treated fruit, the effects of exogenous application of 1-naphthaleneacetic acid (NAA) and abscisic acid (ABA) on chlorophyll and carotenoid accumulation were investigated. The results showed that both ABA and NAA treatments accelerated the color changes from green to orange in the GA and PDJ-treated fruit during the ripening process. With the NAA and ABA treatments, chlorophylls contents were decreased rapidly, and the contents of β,β-xanthophylls were significantly enhanced in the GA and PDJ-treated fruit. In addition, gene expression results showed that the changes of the chlorophyll and carotenoid metabolisms in the NAA and ABA treatments were highly regulated at the transcriptional level. The results presented in this study suggested that the application of NAA and ABA could potentially be used for improving the coloration of the GA and PDJ-treated fruit.
赤霉素（GA）和原脱叶酸（PDJ）联合喷雾是减少温州蜜柑果皮起疱的有效方法。然而，在 GA 和 PDJ 联合处理中，果实的颜色发育在成熟过程中会延迟。在本研究中，为了改善 GA 和 PDJ 处理果实的着色，研究了外源施用萘乙酸（NAA）和脱落酸（ABA）对叶绿素和类胡萝卜素积累的影响。结果表明，ABA 和 NAA 处理均加速了 GA 和 PDJ 处理果实在成熟过程中从绿色向橙色的颜色变化。用 NAA 和 ABA 处理后，GA 和 PDJ 处理果实中的叶绿素含量迅速下降，β，β-叶黄素含量显著增加。此外，基因表达结果表明，NAA 和 ABA 处理中叶绿素和类胡萝卜素代谢的变化在转录水平上受到高度调控。本研究结果表明，NAA 和 ABA 的应用可能有助于改善 GA 和 PDJ 处理果实的着色。
