Department of Endocrinology, The Air Force Hospital from Eastern Theater of PLA, Nanjing, China.
Int Wound J. 2023 May;20(5):1525-1533. doi: 10.1111/iwj.14007. Epub 2022 Nov 4.
Background of the Study Diabetic foot ulcers (DFUs) are severe effect of diabetes. This research aimed to discover the role of micro-ribonucleic acid (miRNA) in treating DFUs involved in maggot debridement therapy (MDT) via a miRNA chip study. A miRNA chip approach was adopted. Patients with diabetes (type 1 or 2) who had at least one-foot ulcer (current or previous) were enrolled in the study. The alterations of miRNA expressions in the granulation tissue during treatment with MDT were measured. Following MDT, the increased expression of miR17-92 was verified in vivo. The miR-17-3p expression increased, and Flk-1 (vascular endothelial growth factor) expression was significantly reduced in patients with DFUs who received MDT (P < 0.01). Results from human umbilical vein endothelial cells that excrete or secrete showed consistency with in vitro findings (P < 0.001, P < 0.05). The overexpression of miR-17-3p demonstrated inhibitory activity on tube formation (P < 0.05). When DFUs were treated with MDT, it revealed that miR-17-3p had a negative regulatory effect on Flk-1.
研究背景
糖尿病足溃疡（DFUs）是糖尿病的严重后果。本研究旨在通过 miRNA 芯片研究发现 miRNA 在蛆清创疗法（MDT）治疗 DFUs 中的作用。采用 miRNA 芯片方法。招募至少有一个足部溃疡（当前或以前）的糖尿病（1 型或 2 型）患者。测量在 MDT 治疗过程中肉芽组织中 miRNA 表达的变化。在 MDT 后，体内验证了 miR17-92 的表达增加。接受 MDT 的 DFU 患者的 miR-17-3p 表达增加，Flk-1（血管内皮生长因子）表达明显降低（P < 0.01）。分泌或分泌的人脐静脉内皮细胞的结果与体外结果一致（P < 0.001，P < 0.05）。miR-17-3p 的过表达对管形成表现出抑制活性（P < 0.05）。当用 MDT 治疗 DFUs 时，发现 miR-17-3p 对 Flk-1 具有负调节作用。
