Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen 361021, PR China.
National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Zhejiang Provincial Key Lab for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, PR China.
Ecotoxicol Environ Saf. 2022 Dec 1;247:114259. doi: 10.1016/j.ecoenv.2022.114259. Epub 2022 Nov 2.
Fluxapyroxad, a succinate dehydrogenase inhibitor (SDHI) fungicide, is commercialized worldwide to control a variety of fungal diseases. Growing evidence shows that fluxapyroxad is teratogenic to aquatic organisms. In this study, the influence of fluxapyroxad toward hematopoietic development was evaluated using zebrafish embryos which were exposed to fluxapyroxad (0.03 µM, 0.3 µM and 3 µM) from 3 h post fertilization (hpf) to 3 days post fertilization (dpf). Compared to the control groups, the hemoglobin was ectopic and decreased in response to fluxapyroxad treatment. The transcription levels of genes (hbbe1, hbbe2, and gata1a) involved in erythropoiesis were reduced after exposure to fluxapyroxad. In contrast, the distributions and expression of marker genes for myeloid lineage cells were unaffected by fluxapyroxad exposure. Our data suggested that fluxapyroxad might specifically affect erythropoiesis and hold great promise for the assessment of the toxicity of fluxapyroxad to aquatic organisms.
氟吡菌酰胺是一种琥珀酸脱氢酶抑制剂（SDHI）杀菌剂，已在全球范围内商业化，用于控制多种真菌病害。越来越多的证据表明，氟吡菌酰胺对水生生物具有致畸性。在这项研究中，通过暴露于氟吡菌酰胺（0.03µM、0.3µM 和 3µM）从受精后 3 小时（hpf）到受精后 3 天（dpf），评估了氟吡菌酰胺对造血发育的影响。与对照组相比，血红蛋白异位且对氟吡菌酰胺处理呈下降趋势。参与红细胞生成的基因（hbbe1、hbbe2 和 gata1a）的转录水平在暴露于氟吡菌酰胺后降低。相比之下，暴露于氟吡菌酰胺对髓系细胞标记基因的分布和表达没有影响。我们的数据表明，氟吡菌酰胺可能特异性地影响红细胞生成，并且在评估氟吡菌酰胺对水生生物的毒性方面具有很大的潜力。
