School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Department of Pharmacy, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
J Pharm Biomed Anal. 2023 Jan 20;223:115130. doi: 10.1016/j.jpba.2022.115130. Epub 2022 Oct 31.
Zhi-Zi-Chi decoction (ZZCD), comprising of Gardenia jasminoides Ellis (GJE) and Semen sojae preparatum (SSP), is a classical Chinese medicine formula. A novel analysis strategy was set up to obtain an evaluation of ZZCD on attenuation and synergy of compatibility. High-resolution ion trap/time-of-flight mass spectrometry (LC-IT-TOF-MS) was used for qualitative analysis. Variant ingredients were analyzed to compare the componential differences between ZZCD formula and single herbs. Based on our previous fingerprint studies that combined with chemometric methods, 13 remarkable chemical markers were selected and evaluated for quantitative determination by high performance liquid chromatography (HPLC) in three different ratios of ZZCD. 62 compounds in ZZCD, 55 compounds in GJE and 16 compounds in SSP were characterized. The compatibility of GJE and SSP may lead to the undetection of hepatotoxic components such as genipin and the emergence of protective components such as jasminoside A, which was not found in single herbs. Meanwhile, 13 selected chemical markers were successfully determined in three ratios of ZZCD. The compatibility may lead to the decrease of toxic ingredients and the increase of beneficial ingredients. By comparing the dissolution of chemical markers, iridoids in GJE and flavonoids in SSP had the best dissolution when the compatibility ratio was 1:1. This strategy would be a valuable reference for further study on the compatibility of traditional Chinese medicine formula.
栀子豉汤（ZZCD）由栀子（GJE）和大豆黄卷（SSP）组成，是一种经典的中药方剂。建立了一种新的分析策略，以评估 ZZCD 在减毒和协同配伍方面的作用。采用高分辨离子阱/飞行时间质谱（LC-IT-TOF-MS）进行定性分析。分析了变体成分，以比较 ZZCD 配方与单味药之间的成分差异。基于我们之前结合化学计量学方法的指纹图谱研究，选择了 13 个显著的化学标志物，并通过高效液相色谱（HPLC）在三种不同比例的 ZZCD 中进行定量测定。在 ZZCD 中鉴定出 62 种化合物，在 GJE 中鉴定出 55 种化合物，在 SSP 中鉴定出 16 种化合物。GJE 和 SSP 的配伍可能导致如京尼平苷和栀子苷 A 等有毒成分的检测不到，同时出现如保护成分等。同时，在三种比例的 ZZCD 中成功测定了 13 个选定的化学标志物。配伍可能导致有毒成分的减少和有益成分的增加。通过比较化学标志物的溶解情况，当配伍比例为 1:1 时，GJE 中的环烯醚萜和 SSP 中的黄酮类化合物具有最佳的溶解性能。该策略将为进一步研究中药方剂的配伍提供有价值的参考。
