Liu Han, Wang Chao, Sun Xiaoqian, Zhan Chaojun, Li Zixiao, Qiu Lin, Luo Rui, Liu Hao, Sun Xiaodi, Li Ruixin, Zhang Jun
Tianjin Stomatological Hospital, The Affiliated Stomatological Hospital of Nankai University, Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin 300041, China.
School of Medicine, Nankai University, Tianjin 300071, China.
ACS Biomater Sci Eng. 2022 Dec 12;8(12):5245-5256. doi: 10.1021/acsbiomaterials.2c00690. Epub 2022 Nov 6.
The fast osteogenesis of the large alveolar fossa and the maintenance of the height of the alveolar ridge after tooth extraction have always been a clinical challenge. Therefore, this work describes the creation of innovative silk fibroin/collagen/hydroxyapatite (SCH) biological scaffolds by 3D printing technology, which are loaded with recombinant human erythropoietin (rh-EPO) for the reconstruction of bone defects. Low-temperature 3D printing can maintain the biological activity of silk fibroin and collagen. The SCH scaffolds showed the ideal water absorption and porosity, being a sustained-release carrier of rh-EPO. The optimized scaffolds had ideal mechanical properties , and MC3T3-E1 cells could easily adhere and proliferate on it. experiments in rabbits demonstrated that the composite scaffolds gradually degraded and promoted the accumulation and proliferation of osteoblasts and the formation of collagen fibers, significantly promoting the reconstruction of mandibular defects. In this study, a novel composite biological scaffold was prepared using 3D printing technology, and the scaffold was innovatively combined with the multifunctional growth factor rh-EPO. This provides a new optimized composite material for the reconstruction of irregular mandible defects, and this biomaterial is promising for clinical reconstruction of alveolar bone defects.
大牙槽窝的快速成骨以及拔牙后牙槽嵴高度的维持一直是一项临床挑战。因此，这项工作描述了通过3D打印技术制备创新的丝素蛋白/胶原蛋白/羟基磷灰石（SCH）生物支架，其负载重组人促红细胞生成素（rh-EPO）用于骨缺损的重建。低温3D打印可以维持丝素蛋白和胶原蛋白的生物活性。SCH支架表现出理想的吸水性和孔隙率，是rh-EPO的缓释载体。优化后的支架具有理想的力学性能，MC3T3-E1细胞能够轻松地在其上黏附并增殖。在兔子身上进行的实验表明，复合支架逐渐降解，促进成骨细胞的聚集和增殖以及胶原纤维的形成，显著促进下颌骨缺损的重建。在本研究中，使用3D打印技术制备了一种新型复合生物支架，并且该支架创新性地与多功能生长因子rh-EPO相结合。这为不规则下颌骨缺损的重建提供了一种新的优化复合材料，这种生物材料在牙槽骨缺损的临床重建方面具有广阔前景。
