de Freitas Karoline Soares, da Silva Lucas Henrique Domingos, Squarisi Iara Silva, de Souza Oliveira Lucas Teixeira, Ribeiro Arthur Barcelos, Alves Bianca Silva, Esperandim Tábata Rodrigues, de Melo Matheus Reis Santos, Ozelin Saulo Duarte, Lemes Danieli Cristina, Bastos Jairo Kenupp, Veneziani Rodrigo Cassio Sola, Tavares Denise Crispim
Mutagenesis Laboratory, University of Franca, 201 Dr Armando Salles de Oliveira Ave, Parque Universitário, Franca, São Paulo 14404-600, Brazil.
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café Ave, Vila Monte Alegre, Ribeirão Preto, São Paulo 14040-900, Brazil.
Toxicol Res (Camb). 2022 Aug 23;11(5):750-757. doi: 10.1093/toxres/tfac049. eCollection 2022 Oct.
Red propolis is synthetized from exudates of (L) Taub. and L.f., presents isoflavones, guttiferone E, xanthochymol, and oblongifolin B and has anti-inflammatory, antioxidant, and antiproliferative activities.
This study aimed to evaluate the antigenotoxic and anticarcinogenic potential of red propolis hydroalcoholic extract (RPHE) in rodents.
The influence of RPHE in doxorubicin (DXR)-induced genotoxicity was investigated through the micronucleus test in Swiss mice. Blood samples were also collected to investigate oxidative stress, hepatotoxicity, and nephrotoxicity. Was investigated the influence of RPHE in 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci, as well as its influence in prolifera cell nuclear antigen (PCNA) and the cyclooxygenase-2 (COX-2) expression in colon of rats, by immunohistochemistry.
The results showed that RPHE (48 mg/kg) reduced DXR-induced genotoxicity. Animals treated with DXR showed significantly lower GSH serum levels in comparison to the negative control. RPHE treatments did not attenuated significantly the DXR-induced GSH depletion. No difference was observed in cytotoxicity parameters of mice hematopoietic tissues between the treatment groups, as well as the biochemical parameters of hepatotoxicity and nephrotoxicity. RPHE (12 mg/kg) reduced the DMH-induced carcinogenicity and toxicity, as well as DMH-induced PCNA and COX-2 expression in colon tissue.
Therefore, was observed that the RPHE has chemopreventive effect, associated to antiproliferative and anti-inflammatory activities.
红蜂胶由（L）陶布和L.f.的分泌物合成，含有异黄酮、藤黄双黄酮E、山柑子醇和长叶烯B，具有抗炎、抗氧化和抗增殖活性。
本研究旨在评估红蜂胶水醇提取物（RPHE）对啮齿动物的抗遗传毒性和抗癌潜力。
通过瑞士小鼠微核试验研究RPHE对阿霉素（DXR）诱导的遗传毒性的影响。还采集血样以研究氧化应激、肝毒性和肾毒性。通过免疫组织化学研究RPHE对1,2-二甲基肼（DMH）诱导的异常隐窝灶的影响，以及其对大鼠结肠增殖细胞核抗原（PCNA）和环氧合酶-2（COX-2）表达的影响。
结果表明，RPHE（48mg/kg）降低了DXR诱导的遗传毒性。与阴性对照相比，用DXR处理的动物血清谷胱甘肽水平显著降低。RPHE处理并未显著减轻DXR诱导的谷胱甘肽消耗。各治疗组小鼠造血组织的细胞毒性参数以及肝毒性和肾毒性的生化参数均未观察到差异。RPHE（12mg/kg）降低了DMH诱导的致癌性和毒性，以及DMH诱导的结肠组织中PCNA和COX-2的表达。
因此，观察到RPHE具有化学预防作用，与抗增殖和抗炎活性相关。
