Laboratory of Pharmacognosy, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Av. do Café S/N, Ribeirão Preto, SP 14040-930, Brazil.
Laboratory of Mutagenesis, University of Franca, Avenida Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca, SP 14404-600, Brazil.
Chem Res Toxicol. 2021 Apr 19;34(4):1024-1033. doi: 10.1021/acs.chemrestox.0c00356. Epub 2021 Mar 15.
Propolis is one of the most widely used products in traditional medicine. One of the most prominent types of Brazilian propolis is the red one, whose primary botanical source is (L.) Taub. Despite the potential of Brazilian red propolis for developing new products with pharmacological activity, few studies guarantee safety in its use. The objective of this study was the evaluation of the possible toxic effects of Brazilian red propolis and . , as well as the cytotoxicity assessment of the main compounds of red propolis on tumoral cell lines. Hydroalcoholic extracts of the Brazilian red propolis (BRPE) and . stems (DSE) and leaves (DLE) were prepared and chromatographed for isolation of the major compounds. RP-HPLC-DAD was used to quantify the major compounds in the obtained extracts. The XTT assay was used to evaluate the cytotoxic activity of the extracts in the human fibroblast cell line (GM07492A). The results revealed IC values of 102.7, 143.4, and 253.1 μg/mL for BRPE, DSE, and DLE, respectively. The extracts were also evaluated for their genotoxic potential in the micronucleus assay in Chinese hamster lung fibroblasts cells (V79), showing the absence of genotoxicity. The BRPE was investigated for its potential toxicity in the zebrafish model. Concentrations of 0.8-6.3 mg/L were safe for the animals, with a LC of 9.37 mg/L. Of the 11 compounds isolated from BRPE, medicarpin showed a selective cytotoxic effect against the HeLa cell line. These are the initial steps to determine the toxicological potential of Brazilian red propolis.
蜂胶是传统医学中应用最广泛的产品之一。巴西蜂胶中最突出的一种是红蜂胶，其主要植物来源是 （L.）Taub。尽管巴西红蜂胶具有开发具有药理活性的新产品的潜力，但很少有研究能保证其使用的安全性。本研究的目的是评估巴西红蜂胶和. 的潜在毒性作用，以及红蜂胶主要化合物对肿瘤细胞系的细胞毒性评估。制备了巴西红蜂胶（BRPE）和. 茎（DSE）和叶（DLE）的水醇提取物，并进行色谱分离以分离主要化合物。反相高效液相色谱-二极管阵列检测（RP-HPLC-DAD）用于测定所得提取物中主要化合物的含量。XTT 测定法用于评估提取物对人成纤维细胞系（GM07492A）的细胞毒性活性。结果表明，BRPE、DSE 和 DLE 的 IC 值分别为 102.7、143.4 和 253.1 μg/mL。还评估了提取物在中华仓鼠肺成纤维细胞（V79）中的微核试验中的遗传毒性潜力，结果显示无遗传毒性。研究了 BRPE 在斑马鱼模型中的潜在 毒性。浓度为 0.8-6.3 mg/L 时对动物安全，LC 为 9.37 mg/L。从 BRPE 中分离出的 11 种化合物中，桑色烯显示出对 HeLa 细胞系的选择性细胞毒性作用。这些是确定巴西红蜂胶毒理学潜力的初步步骤。
