Qian Hengwei, Song Limin, Wang Lulu, Wang Baoshan, Liang Wenxing
College of Life Sciences, Shandong Normal University, Jinan, China.
Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Front Microbiol. 2022 Oct 19;13:1040302. doi: 10.3389/fmicb.2022.1040302. eCollection 2022.
The secretion of peptidases from several pathogens has been reported, but the biological function of these proteins in plant-pathogen interactions is poorly understood. , a soil-borne plant pathogenic fungus that causes wilt in its host, can secrete proteins into host plant cells during the infection process to interfere with the host plant defense response and promote disease occurrence. In this study, we identified a peptidase, FoAPY1, that could be secreted from depending on the N-terminal signal peptide of the protein. FoAPY1 belongs to the peptidase M28 family and exerts peptidase activity . Furthermore, the gene knockout strain (∆FoAYP1) presented reduced virulence to tomato plants, but its mycelial growth and conidiation were unchanged. Moreover, FoAYP1 overexpression tomato seedlings exhibited enhanced susceptibility to and strains. These data demonstrated that FoAYP1 contributes to the virulence of may through peptidase activity against host plant proteins.
已有报道称几种病原体可分泌肽酶，但这些蛋白质在植物与病原体相互作用中的生物学功能却知之甚少。尖孢镰刀菌（Fusarium oxysporum）是一种土传植物病原真菌，可在感染过程中将蛋白质分泌到宿主植物细胞中，干扰宿主植物的防御反应并促进病害发生。在本研究中，我们鉴定出一种肽酶FoAPY1，它可根据该蛋白质的N端信号肽从尖孢镰刀菌中分泌出来。FoAPY1属于肽酶M28家族，并具有肽酶活性。此外，该基因敲除菌株（∆FoAYP1）对番茄植株的毒力降低，但其菌丝生长和分生孢子形成未发生变化。此外，FoAYP1过表达的番茄幼苗对尖孢镰刀菌菌株表现出更高的易感性。这些数据表明，FoAYP1可能通过对宿主植物蛋白质的肽酶活性来促进尖孢镰刀菌的毒力。
