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用于细胞引导应用的羊毛角蛋白纳米颗粒基微图案

Wool Keratin Nanoparticle-Based Micropatterns for Cellular Guidance Applications.

作者信息

Trojanowska Dagmara J, Suarato Giulia, Braccia Clarissa, Armirotti Andrea, Fiorentini Fabrizio, Athanassiou Athanassia, Perotto Giovanni

机构信息

Istituto Italiano di Tecnologia, Smart Materials Group, Via Morego, 30, 16163Genova, Italy.

Department of Materials Science, University of Milano-Bicocca, via R. Cozzi 55, 20125Milan, Italy.

出版信息

ACS Appl Nano Mater. 2022 Oct 28;5(10):15272-15287. doi: 10.1021/acsanm.2c03116. Epub 2022 Oct 4.

DOI:10.1021/acsanm.2c03116
PMID:36338329
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9624257/
Abstract

The waste stream of low-grade wool is an underutilized source of keratin-rich materials with appropriate methods for upcycling into high value-added products still being an open challenge. In the present work, keratins were precipitated from their water solution to produce hierarchical keratin particles via isoelectric precipitation. Matrix-assisted laser desorption/ionization coupled with time-of-flight tandem mass spectrometry analysis (MALDI-TOF/TOF MS/MS) showed the presence of the amino acid sequence leucine-aspartic acid-valine (LDV) in the extracted keratin. This well-known cell adhesion motif is recognized by the cell adhesion molecule αβ integrin. We showed that keratin particles had this tripeptide exposed on the surface and that it could be leveraged, via patterns obtained with microcontact printing, to support and facilitate dermal fibroblast cell adhesion and direct their growth orientation. The zeta potential, isoelectric point, morphological structures, chemical composition, and biocompatibility of keratin particles and the influence of the surfactant sodium dodecyl sulfate (SDS) were investigated. An appropriate ink for microcontact printing of the keratin particles was developed and micron-sized patterns were obtained. Cells adhered preferentially to the patterns, showing how this strategy could be used to functionalize biointerfaces.

摘要

低等级羊毛的废物流是一种未充分利用的富含角蛋白的材料来源，采用适当方法将其升级转化为高附加值产品仍然是一个有待解决的挑战。在本研究中，通过等电沉淀法从角蛋白水溶液中沉淀出角蛋白，以制备分级角蛋白颗粒。基质辅助激光解吸/电离结合飞行时间串联质谱分析（MALDI-TOF/TOF MS/MS）表明，提取的角蛋白中存在氨基酸序列亮氨酸-天冬氨酸-缬氨酸（LDV）。这种著名的细胞黏附基序可被细胞黏附分子αβ整合素识别。我们发现角蛋白颗粒表面暴露有这种三肽，并且可以通过微接触印刷获得的图案来利用它，以支持和促进真皮成纤维细胞的黏附并引导其生长方向。研究了角蛋白颗粒的ζ电位、等电点、形态结构、化学成分和生物相容性以及表面活性剂十二烷基硫酸钠（SDS）的影响。开发了一种用于角蛋白颗粒微接触印刷的合适油墨，并获得了微米级图案。细胞优先黏附于这些图案，展示了这种策略如何用于生物界面的功能化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/f562546a6954/an2c03116_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/4af93a0c6361/an2c03116_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/6bc01ec77d26/an2c03116_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/4d4bba049675/an2c03116_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/58330b9bae76/an2c03116_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/cfbcd60772a3/an2c03116_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/07d50d27ef55/an2c03116_0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/ae902aa3118e/an2c03116_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/d62d66fd04bb/an2c03116_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/fbf359b884fd/an2c03116_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/f562546a6954/an2c03116_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/4af93a0c6361/an2c03116_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/6bc01ec77d26/an2c03116_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/4d4bba049675/an2c03116_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/58330b9bae76/an2c03116_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/cfbcd60772a3/an2c03116_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/07d50d27ef55/an2c03116_0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/ae902aa3118e/an2c03116_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/d62d66fd04bb/an2c03116_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/fbf359b884fd/an2c03116_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bce/9624257/f562546a6954/an2c03116_0010.jpg

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