Department of Food and Nutrition, BioNanocomposite Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Department of Food and Nutrition, BioNanocomposite Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Mater Sci Eng C Mater Biol Appl. 2019 Dec;105:110068. doi: 10.1016/j.msec.2019.110068. Epub 2019 Aug 9.
Eco-friendly antimicrobial nanoparticles were prepared by adsorbing metal ions to keratin nanoparticles (KNP) isolated from the chicken feather. The mean size of KNP determined by STEM and DLS was 47 ± 15 nm and 80.7 ± 8.3 nm, respectively. The KNP-Ag complex exhibited a larger negative surface charge (-28.2 ± 4.2 mV) than the KNP-Cu (-2.7 ± 0.9 mV) and KNP-Zn (-5.7 ± 1.3 mV) complexes. The FTIR results showed that the interaction of KNP and metal ions was different depending on the type of metal ion. EDX and XPS results revealed the presence of metal ions in KNP-metal ion complexes. Metal ion adsorption and desorption kinetics results showed that the silver binding to KNP was the highest among the metal ions tested, while the release rate was the slowest. The adsorption of cationic metal ions promoted the adhesion of KNP to bacterial cell and enhanced the killing effect for both Gram-positive and Gram-negative bacteria. Metal ion depletion test result revealed that the bactericidal activity of the KNP-metal ion complex was time-dependent due to the desorption of metal ions from the KNP core. The antimicrobial activity of the KNP-metal ion complex was dependent on the type of metal ions. The nanoparticle complex prepared by adsorbing antimicrobial metal ions on biodegradable core materials is likely to be used for the development of eco-friendly antimicrobial nanomaterials.
从鸡毛中分离得到角蛋白纳米粒子（KNP），然后将金属离子吸附到 KNP 上，制备出环保型抗菌纳米粒子。通过 STEM 和 DLS 测定的 KNP 的平均粒径分别为 47±15nm 和 80.7±8.3nm。KNP-Ag 复合物的表面负电荷（-28.2±4.2mV）比 KNP-Cu（-2.7±0.9mV）和 KNP-Zn（-5.7±1.3mV）复合物的更大。FTIR 结果表明，KNP 与金属离子的相互作用因金属离子的类型而异。EDX 和 XPS 结果表明，KNP-金属离子复合物中存在金属离子。金属离子吸附和解吸动力学结果表明，在所测试的金属离子中，银与 KNP 的结合最高，而释放速率最慢。阳离子金属离子的吸附促进了 KNP 与细菌细胞的粘附，并增强了对革兰氏阳性菌和革兰氏阴性菌的杀伤作用。金属离子耗尽测试结果表明，由于金属离子从 KNP 核心解吸，KNP-金属离子复合物的杀菌活性是时间依赖性的。KNP-金属离子复合物的抗菌活性取决于金属离子的类型。将抗菌金属离子吸附到可生物降解的核心材料上制备的纳米粒子复合物可能用于开发环保型抗菌纳米材料。
