Tuberculosis Prevention and Control Key Laboratory/Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Senior Department of Tuberculosis, The Eighth Medical Center of PLA General Hospital, Beijing 100091, China.
Department of Geriatrics, The Eighth Medical Center of PLA General Hospital, Beijing 100091, China.
J Immunol Res. 2022 Oct 26;2022:3337622. doi: 10.1155/2022/3337622. eCollection 2022.
Several studies have shown that lymphocyte subsets can mediate the occurrence of osteoporosis (OP); however, the predictive ability of lymphocyte subsets in senile OP has not been elucidated.
To investigate the ability of lymphocyte subsets to predict senile osteoporosis (OP).
This study included 44 patients with senile OP and 44 without OP. Dual-energy X-ray absorptiometry (DEXA) was used to determine bone mineral density (BMD). Flow cytometry was used to analyze the absolute counts of the lymphocyte subsets and cytokine levels. Finally, the correlation between BMD and lymphocyte subset counts in the two groups was analyzed.
There were no significant differences in age, sex, or weight between the OP and non-OP groups. The absolute counts of total T lymphocytes and CD8 T lymphocytes in the OP group were significantly lower than those in the non-OP group. The levels of IFN- or TNF- in the OP group were significantly higher or lower, respectively, than those in the non-OP group. PCA showed that age, BMI, total T lymphocytes, CD4 T lymphocytes, CD8 T lymphocytes, and B lymphocytes were the principal components of senile OP. The linear regression equation showed that BMD of the right femoral neck significantly decreased with a decline in CD8 T lymphocyte counts.
BMD decreased with a decrease in CD8 T lymphocytes. The mechanism by which lower lymphocyte subsets lead to lower BMD may be related to abnormal bone metabolism caused by immune aging. Therefore, we considered that CD8 T lymphocytes could be used to predict the incidence of senile OP.
多项研究表明淋巴细胞亚群可介导骨质疏松症（OP）的发生，然而，淋巴细胞亚群在老年性 OP 中的预测能力尚未阐明。
探讨淋巴细胞亚群预测老年性骨质疏松症（OP）的能力。
本研究纳入 44 例老年性 OP 患者和 44 例非 OP 患者。双能 X 线吸收法（DEXA）用于测定骨密度（BMD）。流式细胞术用于分析淋巴细胞亚群和细胞因子水平的绝对计数。最后，分析两组患者 BMD 与淋巴细胞亚群计数之间的相关性。
OP 组与非 OP 组在年龄、性别或体重方面无显著差异。OP 组总 T 淋巴细胞和 CD8 T 淋巴细胞的绝对计数明显低于非 OP 组。OP 组 IFN-或 TNF-的水平分别明显高于或低于非 OP 组。PCA 显示年龄、BMI、总 T 淋巴细胞、CD4 T 淋巴细胞、CD8 T 淋巴细胞和 B 淋巴细胞是老年性 OP 的主要成分。线性回归方程显示，右侧股骨颈 BMD 随 CD8 T 淋巴细胞计数的下降而显著降低。
BMD 随 CD8 T 淋巴细胞的减少而降低。较低的淋巴细胞亚群导致较低 BMD 的机制可能与免疫衰老引起的异常骨代谢有关。因此，我们认为 CD8 T 淋巴细胞可用于预测老年性 OP 的发生。
