Jafarian Hadis, Amanati Ali, Badiee Parisa
Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Curr Med Mycol. 2022 Mar;8(1):26-31. doi: 10.18502/cmm.8.1.9211.
Taurolidine is active against a wide variety of micro-organisms, including bacteria and fungi. Mucormycosis is one of the life-threatening opportunistic fungal infections, especially in immunocompromised patients. Currently, the emergence of Mucormycosis during the COVID-19 pandemic raises public health concerns regarding untoward morbidity and mortality among SARS-CoV-2 patients. It is well-known that delayed and inappropriate antifungal therapy leads to increased morbidity and mortality. This study aimed to investigate the antifungal activity of taurolidine to evaluate its effects against clinical isolates of .
This study included previously collected clinical isolates. The minimum inhibitory concentration (MIC) of amphotericin B, caspofungin, voriconazole, posaconazole, and itraconazole was determined using the broth microdilution method.
All clinical isolates showed full sensitivity to amphotericin B. Posaconazole MIC range from 8 μg/mL to 0.032 μg/mL. The MIC range of voriconazole and caspofungin were determined to be 2-8 µg/mL and 0.5-16 µg/mL, respectively. Growth of the isolates was entirely inhibited in 1000 µg/mL concentration of taurolidine. In microscopic observations, morphological effects on hyphal growth were observed at 500 µg/mL concentration.
In conclusion, this is an updated experience of using taurolidine against . However, our in-vitro findings need to be confirmed in well-designed clinical trials aimed at treating invasive Mucormycosis infections.
牛磺罗定对多种微生物具有活性，包括细菌和真菌。毛霉病是一种危及生命的机会性真菌感染，尤其是在免疫功能低下的患者中。目前，在新冠疫情期间毛霉病的出现引发了公众对新冠患者不良发病率和死亡率的健康担忧。众所周知，延迟和不适当的抗真菌治疗会导致发病率和死亡率增加。本研究旨在调查牛磺罗定的抗真菌活性，以评估其对临床分离株的作用。
本研究纳入了先前收集的临床分离株。采用肉汤微量稀释法测定两性霉素B、卡泊芬净、伏立康唑、泊沙康唑和伊曲康唑的最低抑菌浓度（MIC）。
所有临床分离株对两性霉素B均表现出完全敏感性。泊沙康唑的MIC范围为8μg/mL至0.032μg/mL。伏立康唑和卡泊芬净的MIC范围分别确定为2 - 8μg/mL和0.5 - 16μg/mL。在1000μg/mL浓度的牛磺罗定中，分离株的生长被完全抑制。在显微镜观察中，在500μg/mL浓度下观察到对菌丝生长的形态学影响。
总之，这是使用牛磺罗定对抗……的最新经验。然而，我们的体外研究结果需要在旨在治疗侵袭性毛霉病感染的精心设计的临床试验中得到证实。
