Bioactive characterization and optimization of seed protein hydrolysate: technology and application of natural enzymes.

Tamarind seeds are one of the major sources of different nutrients and minerals and are used as antioxidant components in various food products. Bioactive peptides are released by enzymatic hydrolysis of major food proteins followed by different separation techniques to isolate them from the mixture of other components. Further incorporation of bioactive peptides in various food products may lead to more diversification, fulfilling different nutritional requirements, and promoting health benefits. The objective of this study was to optimize the parameters for the formation of tamarind protein hydrolysates with the use of pepsin and trypsin. It was observed that pepsin and trypsin derived hydrolysate showed the maximum degree of hydrolysis and other bioactive characteristics (DPPH activity, reducing power, ABTS activity, and antidiabetic activity) under optimized conditions. High-performance liquid chromatography was performed for amino acid profiling on trypsin derived protein hydrolysate. From this study, pepsin and trypsin derived hydrolysate can be considered as a rich source of natural antioxidants for developing food formulation.