Department of Biological Sciences, Microbiology, Faculty of Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, 163 Horria Ave. El-Shatby, P.O. Box 832, Alexandria, Egypt.
Sci Rep. 2022 Nov 7;12(1):18889. doi: 10.1038/s41598-022-23480-7.
This study aims to investigate bacteria for biodegradation of oil pollutants from oily industrial wastewater to be used as bioremediation tools and to determine the characterization of bioremediation bioassays. A screening bioassay was carried out using six exogenous environmental bacterial strains to degrade oily pollution, which indicated promising clearance of the oily wastewater. Two strains, namely Enterobacter cloacae 279-56 (R4) and Pseudomonas otitis MCC10330 (R19), could successfully eliminate oil content and reasonable removal of the organic load. Results showed that the two promising bacterial candidates (R4 and R19) were selected according to the preliminary screening of the six tested bacteria considered the most efficient for all the tested parameters. The highest Removal Efficiency (Removal Efficiency resulted in Residual levels of total dissolved solids (TDS), biochemical oxygen demand, chemical oxygen demand, and Oil content in the treated oily wastewater effluents are 1940, 171, 131, and 84 mg/l respectively where these results are not within safe discharge limits, except for TDS. Hence, the bioremediation assays were carried out using the mixed culture since it was the most efficient strain for degrading all tested parameters.
本研究旨在调查细菌对含油工业废水中油类污染物的生物降解能力，将其作为生物修复工具，并确定生物修复生物测定的特征。使用六种外源环境细菌菌株进行了筛选生物测定，以降解油污染，结果表明该废水具有良好的净化效果。两种菌株，即阴沟肠杆菌 279-56（R4）和耳炎假单胞菌 MCC10330（R19），可以有效地去除油含量和有机负荷。结果表明，根据对六种测试细菌的初步筛选，选择了两种有前途的细菌候选物（R4 和 R19），这两种细菌被认为是所有测试参数中最有效的。最高去除效率（去除效率导致处理含油废水中的总溶解固体（TDS）、生化需氧量、化学需氧量和油含量的残留水平分别为 1940、171、131 和 84mg/l，这些结果除 TDS 外均不在安全排放限值内。因此，由于混合培养物是降解所有测试参数的最有效菌株，因此进行了生物修复试验。
