Faculty of Science, Department of Biosciences, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Johor, Malaysia.
Environ Sci Pollut Res Int. 2019 Apr;26(11):11113-11125. doi: 10.1007/s11356-019-04334-8. Epub 2019 Feb 21.
Despite being a key Malaysian economic contributor, the oil palm industry generates a large quantity of environmental pollutant known as palm oil mill effluent (POME). Therefore, the need to remediate POME has drawn a mounting interest among environmental scientists. This study has pioneered the application of Meyerozyma guilliermondii with accession number (MH 374161) that was isolated indigenously in accessing its potential to degrade POME. This strain was able to treat POME in shake flask experiments under aerobic condition by utilising POME as a sole source of carbon. However, it has also been shown that the addition of suitable carbon and nitrogen sources has significantly improved the degradation potential of M. guilliermondii. The remediation of POME using this strain resulted in a substantial reduction of chemical oxygen demand (COD) of 72%, total nitrogen of 49.2% removal, ammonical nitrogen of 45.1% removal, total organic carbon of 46.6% removal, phosphate of 60.6% removal, and 92.4% removal of oil and grease after 7 days of treatment period. The strain also exhibited an extracellular lipase activity which promotes better wastewater treatment. Additionally, Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses have specifically shown that M. guilliermondii strain can degrade hydrocarbons, fatty acids, and phenolic compounds present in the POME. Ultimately, this study has demonstrated that M. guilliermondii which was isolated indigenously exhibits an excellent degrading ability. Therefore, this strain is suitable to be employed in the remediation of POME, contributing to a safe discharge of the effluent into the environment.
尽管是马来西亚经济的主要贡献者之一，但油棕产业会产生大量被称为棕榈油厂废水（POME）的环境污染物。因此，环境科学家越来越有兴趣修复 POME。本研究率先应用了本土分离的 Meyerozyma guilliermondii，其登录号为 MH374161，具有降解 POME 的潜力。该菌株能够在摇瓶实验中利用 POME 作为唯一碳源在好氧条件下处理 POME。然而，已经表明，添加合适的碳源和氮源显著提高了 M. guilliermondii 的降解潜力。使用该菌株修复 POME 可将化学需氧量（COD）降低 72%、总氮去除率达到 49.2%、氨氮去除率达到 45.1%、总有机碳去除率达到 46.6%、磷去除率达到 60.6%、油脂去除率达到 92.4%，处理时间为 7 天。该菌株还表现出细胞外脂肪酶活性，可促进更好的废水处理。此外，傅里叶变换红外光谱（FTIR）和气相色谱-质谱联用（GC-MS）分析特别表明，M. guilliermondii 菌株可以降解 POME 中存在的碳氢化合物、脂肪酸和酚类化合物。最终，本研究表明，本土分离的 M. guilliermondii 具有出色的降解能力。因此，该菌株适合用于修复 POME，有助于安全排放废水到环境中。
