Guan Yuanyuan, Wei Zhiyuan, Zhou Luyi, Wang Kaige, Zhang Meng, Song Puwen, Hu Ping, Hu Haiyan, Li Chengwei
School of Life Sciences, Henan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Henan Institute of Science and Technology, Xinxiang 453003, China.
School of Agriculture, Henan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Henan Institute of Science and Technology, Xinxiang 453003, China.
Plants (Basel). 2023 Aug 29;12(17):3096. doi: 10.3390/plants12173096.
MicroRNA (miRNA) plays a crucial role in the interactions between plants and pathogens, and identifying disease-related miRNAs could help us understand the mechanisms underlying plant disease pathogenesis and breed resistant varieties. However, the role of miRNA in wheat defense responses remains largely unexplored. The miR397 family is highly conserved in plants and involved in plant development and defense response. Therefore, the purpose of this study was to investigate the function of tae-miR397 in wheat resistance to powdery mildew. The expression pattern analysis revealed that tae-miR397 expression was higher in young leaves than in other tissues and was significantly decreased in wheat Bainong207 leaves after () infection and chitin treatment. Additionally, the expression of tae-miR397 was significantly down-regulated by salicylic acid and induced under jasmonate treatment. The overexpression of tae-miR397 in common wheat Bainong207 enhanced the wheat's susceptibility to powdery mildew in the seedling and adult stages. The rate of spore germination and mycelial growth in transgenic wheat plants overexpressing tae-miR397 was faster than in the untransformed wild-type plants. The target gene of tae-miR397 was predicted to be a wound-induced protein (Tae-WIP), and the function was investigated. We demonstrated that silencing of via barley-stripe-mosaic-virus-induced gene silencing enhanced wheat's susceptibility to powdery mildew. qRT-PCR indicated that tae-miR397 regulated wheat immunity by controlling pathogenesis-related gene expressions. Moreover, the transgenic plants overexpressing tae-miR397 exhibited more tillers than the wild-type plants. This work suggests that tae-miR397 is a negative regulator of resistance against powdery mildew and has great potential for breeding disease-resistant cultivars.
微小RNA（miRNA）在植物与病原体的相互作用中起着关键作用，鉴定与疾病相关的miRNA有助于我们了解植物病害发病机制并培育抗病品种。然而，miRNA在小麦防御反应中的作用仍 largely unexplored。miR397家族在植物中高度保守，参与植物发育和防御反应。因此，本研究的目的是探究tae-miR397在小麦对白粉病抗性中的功能。表达模式分析表明，tae-miR397在幼叶中的表达高于其他组织，在小麦百农207叶片经（）感染和几丁质处理后显著降低。此外，tae-miR397的表达受水杨酸显著下调，并在茉莉酸处理下被诱导。在普通小麦百农207中过表达tae-miR397增强了小麦在幼苗期和成年期对白粉病的易感性。过表达tae-miR397的转基因小麦植株中孢子萌发率和菌丝生长速度比未转化的野生型植株更快。预测tae-miR397的靶基因是一种伤口诱导蛋白（Tae-WIP），并对其功能进行了研究。我们证明，通过大麦条纹花叶病毒诱导的基因沉默使（）沉默会增强小麦对白粉病的易感性。qRT-PCR表明，tae-miR397通过控制病程相关基因的表达来调节小麦的免疫。此外，过表达tae-miR397的转基因植株比野生型植株分蘖更多。这项工作表明，tae-miR397是对白粉病抗性的负调节因子，在培育抗病品种方面具有巨大潜力。
