用于鉴定从牛乳腺分离出的金黄色葡萄球菌和无乳链球菌的快速乳腺炎检测方法的评估。
Evaluation of the Rapid Mastitis Test for identification of Staphylococcus aureus and Streptococcus agalactiae isolated from bovine mammary glands.
作者信息
Watts J L, Owens W E
机构信息
Mastitis Research Laboratory, Louisiana State University Agricultural Center, Homer 71040.
出版信息
J Clin Microbiol. 1988 Apr;26(4):672-4. doi: 10.1128/jcm.26.4.672-674.1988.
A latex agglutination test system (Rapid Mastitis Test [RMT]; Immucell, Portland, Maine) containing reagents for the identification of Staphylococcus aureus and Streptococcus agalactiae from bovine intramammary infections was evaluated with 527 staphylococcal and 267 streptococcal isolates. The RMT Staphylococcus aureus reagent detected 94.2% of 242 Staphylococcus aureus isolates, 80% of 25 Staphylococcus intermedius isolates, and 42.8% of 21 tube coagulase-positive Staphylococcus hyicus isolates. All Streptococcus agalactiae isolates were correctly identified by the RMT Streptococcus agalactiae reagent. Cross-reactions were observed with one Streptococcus dysgalactiae and three Streptococcus uberis strains. The RMT was found to be an acceptable method for the detection of Staphylococcus aureus and Streptococcus agalactiae isolated from bovine mammary glands. The occurrence of coagulase-positive staphylococci other than Staphylococcus aureus requires biochemical testing for species level identification.
一种乳胶凝集试验系统（快速乳腺炎检测[RMT]；Immucell公司，缅因州波特兰市），其包含用于从牛乳房内感染中鉴定金黄色葡萄球菌和无乳链球菌的试剂，对527株葡萄球菌和267株链球菌分离株进行了评估。RMT金黄色葡萄球菌试剂检测出242株金黄色葡萄球菌分离株中的94.2%、25株中间葡萄球菌分离株中的80%以及21株试管凝固酶阳性猪葡萄球菌分离株中的42.8%。所有无乳链球菌分离株均被RMT无乳链球菌试剂正确鉴定。观察到与1株停乳链球菌和3株乳房链球菌菌株发生交叉反应。发现RMT是检测从牛乳腺分离出的金黄色葡萄球菌和无乳链球菌的可接受方法。除金黄色葡萄球菌外的凝固酶阳性葡萄球菌的出现需要进行生化试验以进行种水平鉴定。
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