环孢素 A 与各种防腐剂和抗病毒药物对腺病毒 8 型（流行角膜结膜炎的常见病因）的体外疗效。
In Vitro efficacy of cyclosporine a and various antiseptics and antiviral drugs on adenovirus genotype 8, a common cause of epidemic keratoconjunctivitis.
机构信息
Department of Ophthalmology, Adana City Training and Research Hospital, Adana, Turkey.
Department of Ophthalmology, School of Medicine, Cukurova University, Adana, Turkey.
出版信息
Int Ophthalmol. 2023 May;43(5):1701-1710. doi: 10.1007/s10792-022-02567-0. Epub 2022 Nov 8.
PURPOSE
To evaluate the in vitro efficacy of cidofovir, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A on adenovirus genotype 8.
METHODS
Conjunctival samples were collected from patients with adenoviral conjunctivitis and cultured in A549 cells. Adenovirus diagnosis was confirmed by RT-PCR. For each drug, the 50% cytotoxic concentration (CC 50 ) was determined. Subsequently, the antiviral activity was tested at concentrations below CC 50, and the 50% inhibitor concentration (IC 50 ) of drugs was determined RESULTS: While the IC 50 of cidofovir against adenovirus genotype 8 was 3.07 ± 0.8 µM, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A were not found to be effective against adenovirus genotype 8 at concentrations below the CC 50 value.
CONCLUSIONS
Cidofovir was found effective and the IC 50 value was within the ranges in the literature. Ganciclovir and cyclosporine A were found to be ineffective at doses below the cytotoxic dose, povidone-iodine and chlorhexidine was found to be highly cytotoxic.
目的
评估西多福韦、更昔洛韦、聚维酮碘、洗必泰、环孢素 A 对腺病毒 8 型的体外疗效。
方法
从腺病毒性结膜炎患者的结膜样本中采集标本，并在 A549 细胞中培养。通过 RT-PCR 确认腺病毒诊断。对于每种药物，确定其 50%细胞毒性浓度（CC50）。然后，在低于 CC50 的浓度下测试抗病毒活性，并确定药物的 50%抑制浓度（IC50）。
结果
西多福韦对腺病毒 8 型的 IC50 为 3.07±0.8 µM，而在低于 CC50 值的浓度下，更昔洛韦、聚维酮碘、洗必泰和环孢素 A 均未发现对腺病毒 8 型有效。
结论
西多福韦被发现有效，IC50 值在文献范围内。更昔洛韦和环孢素 A 在低于细胞毒性剂量的剂量下无效，聚维酮碘和洗必泰被发现具有高度细胞毒性。
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