Department of Ophthalmology, Adana City Training and Research Hospital, Adana, Turkey.
Department of Ophthalmology, School of Medicine, Cukurova University, Adana, Turkey.
Int Ophthalmol. 2023 May;43(5):1701-1710. doi: 10.1007/s10792-022-02567-0. Epub 2022 Nov 8.
To evaluate the in vitro efficacy of cidofovir, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A on adenovirus genotype 8.
Conjunctival samples were collected from patients with adenoviral conjunctivitis and cultured in A549 cells. Adenovirus diagnosis was confirmed by RT-PCR. For each drug, the 50% cytotoxic concentration (CC 50 ) was determined. Subsequently, the antiviral activity was tested at concentrations below CC 50, and the 50% inhibitor concentration (IC 50 ) of drugs was determined RESULTS: While the IC 50 of cidofovir against adenovirus genotype 8 was 3.07 ± 0.8 µM, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A were not found to be effective against adenovirus genotype 8 at concentrations below the CC 50 value.
Cidofovir was found effective and the IC 50 value was within the ranges in the literature. Ganciclovir and cyclosporine A were found to be ineffective at doses below the cytotoxic dose, povidone-iodine and chlorhexidine was found to be highly cytotoxic.
评估西多福韦、更昔洛韦、聚维酮碘、洗必泰、环孢素 A 对腺病毒 8 型的体外疗效。
从腺病毒性结膜炎患者的结膜样本中采集标本，并在 A549 细胞中培养。通过 RT-PCR 确认腺病毒诊断。对于每种药物，确定其 50%细胞毒性浓度（CC50）。然后，在低于 CC50 的浓度下测试抗病毒活性，并确定药物的 50%抑制浓度（IC50）。
西多福韦对腺病毒 8 型的 IC50 为 3.07±0.8 µM，而在低于 CC50 值的浓度下，更昔洛韦、聚维酮碘、洗必泰和环孢素 A 均未发现对腺病毒 8 型有效。
西多福韦被发现有效，IC50 值在文献范围内。更昔洛韦和环孢素 A 在低于细胞毒性剂量的剂量下无效，聚维酮碘和洗必泰被发现具有高度细胞毒性。
