12 号染色体开放阅读框 49 促进结直肠癌肿瘤生长并预测不良预后。
Chromosome 12 Open Reading Frame 49 Promotes Tumor Growth and Predicts Poor Prognosis in Colorectal Cancer.
机构信息
Department of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
出版信息
Dig Dis Sci. 2023 Apr;68(4):1306-1315. doi: 10.1007/s10620-022-07751-x. Epub 2022 Nov 8.
BACKGROUND AND AIMS
Little is known about the role of chromosome 12 open reading frame 49 (C12ORF49)-induced metabolic signal transduction in tumor growth. We investigated the relationship between C12ORF49 expression and prognosis in colorectal cancer (CRC) patients.
METHODS
C12ORF49 protein expression was measured in CRC tissues by Western blot and immunohistochemistry staining. Knock out of C12ORF49 in CRC cells was then performed, and the role of C12ORF49 in CRC cell proliferation and growth was examined. The expression of C12ORF49 in CRC was analyzed in Gene Expression Profiling Interactive Analysis (GEPIA) databases. A prognosis model with 11 C12ORF49-associated genes (CAGs) was generated by TCGA databases.
RESULTS
C12ORF49 expression was significantly higher in CRC tumor tissue than in non-tumor tissue. Furthermore, in vitro and in vivo loss-of-function experiments, showed that C12ORF49 plays critical roles in promoting tumor cell growth. There was a significant correlation between C12ORF49 protein and the presence of tumor necrosis. C12ORF49 is critical for its interaction with SREBF1, TMEM41A, and S1PR3 in the poor prognosis of CRC.
CONCLUSIONS
Our results suggest that C12ORF49 plays a key role in CRC tumor growth.
背景与目的
关于染色体 12 开放阅读框 49（C12ORF49）诱导的代谢信号转导在肿瘤生长中的作用知之甚少。我们研究了 C12ORF49 表达与结直肠癌（CRC）患者预后之间的关系。
方法
通过 Western blot 和免疫组织化学染色检测 CRC 组织中的 C12ORF49 蛋白表达。然后敲除 CRC 细胞中的 C12ORF49，并检测 C12ORF49 在 CRC 细胞增殖和生长中的作用。在基因表达谱交互分析（GEPIA）数据库中分析 CRC 中 C12ORF49 的表达。通过 TCGA 数据库生成包含 11 个 C12ORF49 相关基因（CAGs）的预后模型。
结果
CRC 肿瘤组织中的 C12ORF49 表达明显高于非肿瘤组织。此外，体外和体内功能丧失实验表明，C12ORF49 在促进肿瘤细胞生长中起关键作用。C12ORF49 蛋白与肿瘤坏死的存在之间存在显著相关性。C12ORF49 与其在 CRC 不良预后中的 SREBF1、TMEM41A 和 S1PR3 的相互作用至关重要。
结论
我们的结果表明 C12ORF49 在 CRC 肿瘤生长中发挥关键作用。
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