Zhang Jing, Yi Congmin, Han Jiaojiao, Ming Tinghong, Zhou Jun, Lu Chenyang, Li Ye, Su Xiurong
State Key Laboratory for Quality and Safety of Argo-products Ningbo University Ningbo China.
School of Marine Science Ningbo University Ningbo China.
Food Sci Nutr. 2022 Jul 15;10(11):3814-3827. doi: 10.1002/fsn3.2978. eCollection 2022 Nov.
Aging is closely related to altered gut function and its microbiome composition. To elucidate the mechanisms involved in the preventive effect of special high-docosahexaenoic acid tuna oil (HDTO) on senescence, the effects of different doses of HDTO on the gut microbiome and metabolome of d-galactose-induced aging mice were studied. Deferribacteres and Tenericutes and uridine might be used as indicator bacteria and characteristic metabolites to identify aging, respectively. HDTO markedly improved the impaired memory and antioxidant abilities induced by d-galactose. At the phylum level, the abundance of Firmicutes and Tenericutes was significantly increased upon d-galactose induction, while that of Bacteroidetes, Proteobacteria, and Deferribacteres was significantly decreased. At the genus level, the variation mainly presented as an increase in the abundance of the Firmicutes genera , , and , the decrease in the abundance of the Bacteroidetes genera and , the Firmicutes genus , and the Deferribacteres genus . HDTO supplementation reversed the alterations in the intestinal flora by promoting the proliferation of beneficial flora during the aging process; the metabolic pathways, such as glycine-serine-threonine metabolism, valine-leucine-isoleucine biosynthesis, and some metabolic pathways involved in uridine, were also partially restored. Furthermore, the correlation analysis illustrated an obvious correlation between gut microbiota, its metabolites, and aging-related indices. Moreover, it is worth noting that the metabolic regulation by dietary intervention varied with different HDTO doses and did not present a simple additive effect; indeed, each dose showed a unique modulation mechanism.
衰老与肠道功能及其微生物群组成的改变密切相关。为了阐明特殊的高二十二碳六烯酸金枪鱼油（HDTO）对衰老的预防作用所涉及的机制，研究了不同剂量的HDTO对D-半乳糖诱导的衰老小鼠肠道微生物群和代谢组的影响。脱铁杆菌门和柔膜菌门以及尿苷可能分别用作识别衰老的指示菌和特征性代谢物。HDTO显著改善了D-半乳糖诱导的记忆损伤和抗氧化能力。在门水平上，D-半乳糖诱导后厚壁菌门和柔膜菌门的丰度显著增加，而拟杆菌门、变形菌门和脱铁杆菌门的丰度显著降低。在属水平上，变化主要表现为厚壁菌属、和丰度增加，拟杆菌属和丰度降低，厚壁菌属和脱铁杆菌属丰度降低。补充HDTO通过促进衰老过程中有益菌群的增殖逆转了肠道菌群的改变；甘氨酸-丝氨酸-苏氨酸代谢、缬氨酸-亮氨酸-异亮氨酸生物合成以及一些与尿苷有关的代谢途径也部分恢复。此外，相关性分析表明肠道微生物群、其代谢物与衰老相关指标之间存在明显相关性。此外，值得注意的是，饮食干预的代谢调节因HDTO剂量不同而有所不同，且不存在简单的累加效应；事实上，每个剂量都显示出独特的调节机制。
