School of Marine Science , Ningbo University , Ningbo 315211 , China.
Department of Food Science , Cornell University , Ithaca , New York 14853 , United States.
J Agric Food Chem. 2018 Jun 6;66(22):5510-5520. doi: 10.1021/acs.jafc.8b00446. Epub 2018 Apr 20.
To discern whether tuna oil modulates the expression of brain proteins and the gut microbiota structure during aging induced by d-galactose, we generated an aging mouse model with d-galactose treatment, and the mice showed aging and memory deterioration symptoms according to physiological and biochemical indices. Treatment with different doses of tuna oil alleviated the symptoms; the high dose showed a better effect. Subsequently, brain proteomic analysis showed the differentially expressed proteins were involved in damaged synaptic system repairment and signal transduction system enhancement. In addition, tuna oil treatment restored the diversity of gut microbiota, 27 key operational taxonomic units, which were identified using a redundancy analysis and were significantly correlated with at least one physiological index and three proteins or genes. These findings suggest that the combination of proteomics and gut microbiota is an effective strategy to gain novel insights regarding the effect of tuna oil treatment on the microbiota-gut-brain axis.
为了探究金枪鱼油是否能在半乳糖诱导的衰老过程中调节大脑蛋白的表达和肠道微生物群结构，我们生成了一个衰老小鼠模型，并通过半乳糖处理，使小鼠出现衰老和记忆恶化的症状，这些症状可以通过生理和生化指标来判断。不同剂量的金枪鱼油处理可以缓解这些症状，其中高剂量的效果更好。随后，大脑蛋白质组学分析显示，差异表达的蛋白质涉及受损的突触系统修复和信号转导系统增强。此外，金枪鱼油处理恢复了肠道微生物群的多样性，通过冗余分析鉴定出 27 个关键的操作分类单元，它们与至少一个生理指标以及三种蛋白质或基因显著相关。这些发现表明，蛋白质组学和肠道微生物群的结合是一种有效的策略，可以深入了解金枪鱼油处理对微生物群-肠道-大脑轴的影响。
