C2VN, Aix Marseille Univ, INRAE, INSERM, Faculté de Médecine, 27 boulevard Jean Moulin, Marseille, 13005, France.
Department of Science and Technology, Food and Nutrition Research Institute, Bicutan, Taguig City, NCR 1631, Philippines.
Mol Nutr Food Res. 2023 Jan;67(2):e2200580. doi: 10.1002/mnfr.202200580. Epub 2022 Dec 3.
Phytofluene is a colorless carotenoid with potential health benefits that displays a higher bioavailability compared to carotenoids such as lutein, β-carotene or lycopene. Several studies suggest its bioavailability displays an elevated interindividual variability. The aim of this work is to investigate whether a combination of SNPs is associated with this variability.
Thirty-seven healthy adult males consume a test meal that provides phytofluene from a tomato puree. Phytofluene concentrations are measured at fast and in chylomicrons at regular time intervals after meal intake. Identification of the combination of SNPs that best explained the interindividual variability of the phytofluene response is assessed by partial least squares regression. There is a large interindividual variability in the phytofluene response, with CV = 88%. Phytofluene bioavailability is positively correlated with fasting plasma phytofluene concentration (r = 0.57; p = 2 × 10 ). A robust partial least squares regression model comprising 14 SNPs near or within 11 genes (ABCA1-rs2487059, rs2515629, and rs4149316, APOC1-rs445925, CD36-rs3211881, ELOVL5-rs6941533, FABP1-rs10185660, FADS3-rs1000778, ISX-rs130461, and rs17748559, LIPC-rs17240713, LPL-rs7005359, LYPLAL1-rs1351472, SETD7-rs11936429) explains 51% (adjusted R ) of the interindividual variability in phytofluene bioavailability.
This study reports a combination of SNPs that is associated with a significant part of the interindividual variability of phytofluene bioavailability in a healthy male adult population.
植物虾青素是一种无色类胡萝卜素，具有潜在的健康益处，与叶黄素、β-胡萝卜素或番茄红素等类胡萝卜素相比，具有更高的生物利用度。多项研究表明，其生物利用度显示出个体间的高度可变性。本研究旨在探讨是否存在 SNP 组合与这种变异性相关。
37 名健康成年男性食用了一种提供番茄红素的番茄泥测试餐，在餐后定期时间间隔内测量快进和乳糜微粒中的植物虾青素浓度。通过偏最小二乘回归评估最佳解释植物虾青素反应个体间变异性的 SNP 组合。植物虾青素反应的个体间变异性很大，CV=88%。植物虾青素生物利用度与空腹血浆植物虾青素浓度呈正相关（r=0.57；p=2×10-3）。一个包含 11 个基因附近或内部 14 个 SNP 的稳健偏最小二乘回归模型（ABCA1-rs2487059、rs2515629 和 rs4149316、APOC1-rs445925、CD36-rs3211881、ELOVL5-rs6941533、FABP1-rs10185660、FADS3-rs1000778、ISX-rs130461 和 rs17748559、LIPC-rs17240713、LPL-rs7005359、LYPLAL1-rs1351472、SETD7-rs11936429）解释了植物虾青素生物利用度个体间变异性的 51%（调整后的 R2）。
本研究报告了一组与健康成年男性人群中植物虾青素生物利用度个体间变异性的显著部分相关的 SNP 组合。
