Department of Material Sciences and Engineering, University of Florida, Gainesville, Florida 32611, USA.
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, USA.
Soft Matter. 2022 Nov 23;18(45):8554-8560. doi: 10.1039/d2sm01013e.
In many tissues, cell type varies over single-cell length-scales, creating detailed heterogeneities fundamental to physiological function. To gain understanding of the relationship between tissue function and detailed structure, and eventually to engineer structurally and physiologically accurate tissues, we need the ability to assemble 3D cellular structures having the level of detail found in living tissue. Here we introduce a method of 3D cell assembly having a level of precision finer than the single-cell scale. With this method we create detailed cellular patterns, demonstrating that cell type can be varied over the single-cell scale and showing function after their assembly.
在许多组织中，细胞类型在单细胞长度尺度上存在差异，从而产生了对生理功能至关重要的详细异质性。为了深入了解组织功能与详细结构之间的关系，并最终构建结构和生理上准确的组织，我们需要能够组装具有与活体组织中相同详细程度的 3D 细胞结构。在这里，我们介绍了一种具有优于单细胞尺度精度的 3D 细胞组装方法。使用这种方法，我们创建了详细的细胞图案，证明了可以在单细胞尺度上改变细胞类型，并在组装后显示其功能。
