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SARS-CoV-2 对住院患者的人鼻咽喉微生物群影响有限。

Limited Impact of SARS-CoV-2 on the Human Naso-Oropharyngeal Microbiota in Hospitalized Patients.

机构信息

Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Konggrid.10784.3a, Hong Kong SAR, China.

Department of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Konggrid.10784.3a, Hong Kong SAR, China.

出版信息

Microbiol Spectr. 2022 Dec 21;10(6):e0219622. doi: 10.1128/spectrum.02196-22. Epub 2022 Nov 9.

DOI:10.1128/spectrum.02196-22
PMID:36350127
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9769582/
Abstract

Numerous studies have reported dysbiosis in the naso- and/or oro-pharyngeal microbiota of COVID-19 patients compared with healthy individuals; however, only a few small-scale studies have also included a disease control group. In this study, we characterized and compared the bacterial communities of pooled nasopharyngeal and throat swabs from hospitalized COVID-19 patients ( = 76), hospitalized non-COVID-19 patients with respiratory symptoms or related illnesses ( = 69), and local community controls ( = 76) using 16S rRNA gene V3-V4 amplicon sequencing. None of the subjects received antimicrobial therapy within 2 weeks prior to sample collection. Both COVID-19 and non-COVID-19 hospitalized patients differed in the composition, alpha and beta diversity, and metabolic potential of the naso-oropharyngeal microbiota compared with local controls. However, the microbial communities in the two hospitalized patient groups did not differ significantly from each other. Differential abundance analysis revealed the enrichment of nine bacterial genera in the COVID-19 patients compared with local controls; however, six of them were also enriched in the non-COVID-19 patients. Bacterial genera uniquely enriched in the COVID-19 patients included and . In contrast, and were dramatically decreased in COVID-19 patients only. Association analysis revealed that in COVID-19 patients was positively correlated with the level of the inflammation biomarker C-reactive protein. Our findings reveal a limited impact of SARS-CoV-2 on the naso-oropharyngeal microbiota in hospitalized patients and suggest that and are more specific biomarkers for COVID-19 detection. Our results showed that while both COVID-19 and non-COVID-19 hospitalized patients differed in the composition, alpha and beta diversity, and metabolic potential of the naso-oropharyngeal microbiota compared with local controls, the microbial communities in the two hospitalized patient groups did not differ significantly from each other, indicating a limited impact of SARS-CoV-2 on the naso-oropharyngeal microbiota in hospitalized patients. Besides, we identified and as bacterial genera uniquely enriched in COVID-19 patients, which may serve as more specific biomarkers for COVID-19 detection.

摘要

大量研究报道，与健康个体相比，COVID-19 患者的鼻腔和/或口咽微生物群落存在失调现象；然而，仅有少数小规模研究纳入了疾病对照组。在这项研究中，我们采用 16S rRNA 基因 V3-V4 扩增子测序，对住院 COVID-19 患者（n=76）、患有呼吸道症状或相关疾病的住院非 COVID-19 患者（n=69）和当地社区对照者（n=76）的鼻咽和咽喉抽吸物的细菌群落进行了特征描述和比较。在采集样本前的 2 周内，所有受试者均未接受过抗菌治疗。与当地对照组相比，COVID-19 患者和非 COVID-19 住院患者的鼻咽微生物群的组成、alpha 和 beta 多样性以及代谢潜能均存在差异。然而，两组住院患者的微生物群落彼此之间并无显著差异。差异丰度分析显示，与当地对照组相比，COVID-19 患者中九个细菌属的丰度增加；然而，其中六个细菌属在非 COVID-19 患者中也增加。COVID-19 患者中唯一增加的细菌属包括 和 。相比之下，COVID-19 患者中 和 显著减少。关联分析显示，COVID-19 患者中的 与炎症生物标志物 C 反应蛋白的水平呈正相关。我们的研究结果揭示了 SARS-CoV-2 对住院患者鼻咽微生物群的影响有限，并表明 和 是 COVID-19 检测的更特异性生物标志物。我们的研究结果表明，与当地对照组相比，COVID-19 患者和非 COVID-19 住院患者的鼻咽微生物群的组成、alpha 和 beta 多样性以及代谢潜能存在差异，但两组住院患者的微生物群落之间无显著差异，这表明 SARS-CoV-2 对住院患者的鼻咽微生物群的影响有限。此外，我们发现 和 是 COVID-19 患者中特有的细菌属，可能是 COVID-19 检测的更特异性生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/35a300434063/spectrum.02196-22-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/8fee8f531f09/spectrum.02196-22-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/f6864decb717/spectrum.02196-22-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/17dd04695f6e/spectrum.02196-22-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/316f117feb46/spectrum.02196-22-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/55f0f224eac1/spectrum.02196-22-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/35a300434063/spectrum.02196-22-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/8fee8f531f09/spectrum.02196-22-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/f6864decb717/spectrum.02196-22-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/17dd04695f6e/spectrum.02196-22-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/316f117feb46/spectrum.02196-22-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/55f0f224eac1/spectrum.02196-22-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992b/9769582/35a300434063/spectrum.02196-22-f006.jpg

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