Proderm.
University of Tuebingen, Pharmaceutical Technology, Tuebingen, Germany.
Int J Cosmet Sci. 2023 Feb;45(1):14-28. doi: 10.1111/ics.12820. Epub 2022 Nov 24.
Tape stripping is an often-used non-invasive destructive method to investigate the skin penetration of a substance. In recent years, however, the suitability of confocal Raman spectroscopy (CRS) as a non-invasive method of non-destructive examination of the skin has become increasingly apparent. In this study, we compared invasion and depletion penetration and permeation kinetics of a 2% caffeine solution with and without 1,2-pentanediol as a penetration enhancer measured with CRS and tape stripping.
Porcine skin was used for tape stripping and human skin for CRS. 2% caffeine solution was applied to the skin for different incubation times. Human skin was then examined by CRS while caffeine was extracted from porcine skin and quantified via reverse-phase HPLC. Fluxes were also measured and calculated by sum of the total amounts of caffeine penetrated into the skin.
Without penetration enhancers, there is hardly any difference between the penetration profiles of the two measurement methods for invasion, but the curves for depletion are different. Furthermore, the calculated flux values for the invasion are almost identical, but for the depletion the tape stripping values are about twice as high as the CRS values.
The relevance of conducting invasion and depletion studies became clear and was able to show the still existing problems in the comparability of CRS and tape stripping.
胶带剥离是一种常用的非侵入性破坏性方法，用于研究物质的皮肤渗透。然而，近年来，共聚焦拉曼光谱（CRS）作为一种非侵入性的无损检测皮肤的方法的适用性变得越来越明显。在这项研究中，我们比较了 2%咖啡因溶液和含有 1,2-戊二醇作为渗透增强剂的咖啡因溶液的渗透和耗竭渗透动力学，使用 CRS 和胶带剥离进行测量。
猪皮用于胶带剥离，人皮用于 CRS。将 2%咖啡因溶液应用于皮肤不同的孵育时间。然后用人皮进行 CRS 检查，同时从猪皮中提取咖啡因并通过反相高效液相色谱法进行定量。通量也通过渗透到皮肤中的咖啡因总量进行测量和计算。
没有渗透增强剂时，两种测量方法的入侵渗透曲线几乎没有差异，但耗竭曲线不同。此外，入侵的计算通量值几乎相同，但耗竭时胶带剥离值是 CRS 值的两倍左右。
进行入侵和耗竭研究的相关性变得清晰，并能够显示 CRS 和胶带剥离的可比性中仍然存在的问题。
