Centro de Investigación en Alimentación y Desarrollo, A.C. Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, Mexico.
Instituto de Ciencias del Mar y Limnología, Unidad Académica Mazatlán, Universidad Nacional Autónoma de México, Mazatlán, Sinaloa 82040, Mexico.
Parasite. 2022;29:51. doi: 10.1051/parasite/2022052. Epub 2022 Nov 9.
Genomic resources for Platyhelminthes of the class Monogenea are scarce, despite the diversity of these parasites, some species of which are highly pathogenic to their fish hosts. This work aimed to generate de novo-assembled transcriptomes of two monogenean species, Scutogyrus longicornis (Dactylogyridae) and Rhabdosynochus viridisi (Diplectanidae), providing a protocol for cDNA library preparation with low input samples used in single cell transcriptomics. This allowed us to work with sub-microgram amounts of total RNA with success. These transcriptomes consist of 25,696 and 47,187 putative proteins, respectively, which were further annotated according to the Swiss-Prot, Pfam, GO, KEGG, and COG databases. The completeness values of these transcriptomes evaluated with BUSCO against Metazoa databases were 54.1% and 73%, respectively, which is in the range of other monogenean species. Among the annotations, a large number of terms related to G-protein-coupled receptors (GPCRs) were found. We identified 109 GPCR-like sequences in R. viridisi, and 102 in S. longicornis, including family members specific for Platyhelminthes. Rhodopsin was the largest family according to GRAFS classification. Two putative melatonin receptors found in S. longicornis represent the first record of this group of proteins in parasitic Platyhelminthes. Forty GPCRs of R. viridisi and 32 of S. longicornis that were absent in Vertebrata might be potential drug targets. The present study provides the first publicly available transcriptomes for monogeneans of the subclass Monopisthocotylea, which can serve as useful genomic datasets for functional genomic research of this important group of parasites.
盘形动物门的单殖吸虫类的基因组资源非常有限，尽管这些寄生虫的种类繁多，其中一些对鱼类宿主具有高度致病性。本研究旨在生成两种单殖吸虫（指环虫科的 S. longicornis 和双鳞盘虫科的 Rhabdosynochus viridisi）的从头组装转录组，为使用单细胞转录组学的低输入样本制备 cDNA 文库提供了方案。这使得我们能够成功地使用亚微克量的总 RNA 进行工作。这些转录组分别包含 25696 和 47187 个推定蛋白，根据 Swiss-Prot、Pfam、GO、KEGG 和 COG 数据库进行了进一步注释。使用 BUSCO 对后生动物数据库评估这些转录组的完整性值分别为 54.1%和 73%，这在其他单殖吸虫物种的范围内。在注释中，发现了大量与 G 蛋白偶联受体（GPCR）相关的术语。我们在 R. viridisi 中鉴定出 109 个 GPCR 样序列，在 S. longicornis 中鉴定出 102 个，包括特定于扁形动物的家族成员。Rhodopsin 根据 GRAFS 分类是最大的家族。在 S. longicornis 中发现的两种假定的褪黑素受体代表了该组蛋白质在寄生扁形动物中的首次记录。R. viridisi 中缺失的 40 个 GPCR 和 S. longicornis 中缺失的 32 个 GPCR 可能是潜在的药物靶点。本研究提供了首例可公开获得的单殖吸虫亚类 Monopisthocotylea 的转录组，可为该重要寄生虫群的功能基因组研究提供有用的基因组数据集。
