Ray D S, Kook K
Gene. 1978 Oct;4(2):109-19. doi: 10.1016/0378-1119(78)90024-0.
The transposable genetic element Tn3, which carries an ampicillin (Ap) resistance determinant, has been translocated from a ColE1-Apr plasmid, RSF2124, to the genome of the filamentous single-stranded DNA phage M13. The site orientation of the inserted element has been determined for one such phage, M13::Tn3-15. The insertion is within the intergenic space separating genes 2 and 4 and containing both the viral strand and complementary strand origins. The lengths of both the filamentous phage and the duplex replicative form (RF) DNA are 1.7--1.8 times those of M13 phage and replicative form DNA. Both plaque formation and transduction of sensitive cells to ampicillin resistance by M13::Tn3-15 are sensitive to purified antibodies to the M13 major coat protein.
携带氨苄青霉素（Ap）抗性决定簇的转座遗传元件Tn3已从ColE1 - Apr质粒RSF2124转移至丝状单链DNA噬菌体M13的基因组中。已确定了一个这样的噬菌体M13::Tn3 - 15中插入元件的位点方向。插入发生在分隔基因2和基因4的基因间区域内，该区域包含病毒链和互补链的起始位点。丝状噬菌体和双链复制型（RF）DNA的长度均为M13噬菌体和复制型DNA长度的1.7 - 1.8倍。M13::Tn3 - 15形成噬菌斑以及将敏感细胞转导为氨苄青霉素抗性均对针对M13主要外壳蛋白的纯化抗体敏感。
