National Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals, GRINM Group Co., Ltd., Beijing, 101407, China.
School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Sci Rep. 2022 Nov 9;12(1):19053. doi: 10.1038/s41598-022-21356-4.
Reaction kinetics in biodegradation process is the basis and key of bioremediation technology, which can be used to predict the interaction between microorganisms and environmental states in the system. In this study, the kinetic model (Monod, Moser, Tessier and Cotonis) and kinetic parameters of aerobic biodegradation of functional bacteria in simulated wastewater polluted by aromatic volatile organic compounds (VOCs) were determined by shaking flask experiment. Monod, Moser, Tessier and Contois models were used to fit the experimental data and determine the kinetic parameters based on nonlinear regression analysis. Experimental results demonstrated that the removal rate of aromatic VOCs at 72 h was between 34.78 and 99.75% depending on the initial concentration of aromatic VOCs. The specific growth rate μ and degradation rate q increased with the increase of substrate concentration. The model of Monod, Moser and Tessier could be used to simulate microbial growth and substrate degradation in simulated aromatic VOCs polluted wastewater. Then the model and corresponding kinetic parameters were used to predict the limit concentration of biodegradation and provide theoretical support for the subsequent dynamic simulation and field engineering.
在生物修复技术中，生物降解过程中的反应动力学是基础和关键，可以用来预测系统中微生物与环境状态之间的相互作用。本研究采用摇瓶实验，确定了模拟芳香族挥发性有机化合物（VOCs）污染废水的功能菌好氧生物降解的动力学模型（Monod、Moser、Tessier 和 Cotonis）和动力学参数。采用 Monod、Moser、Tessier 和 Contois 模型对实验数据进行拟合，通过非线性回归分析确定动力学参数。实验结果表明，在 72 h 时，根据芳香族 VOCs 的初始浓度，芳香族 VOCs 的去除率在 34.78%~99.75%之间。比生长速率μ和降解速率 q随基质浓度的增加而增加。Monod、Moser 和 Tessier 模型可用于模拟模拟芳香族 VOCs 污染废水中微生物的生长和基质的降解。然后使用该模型和相应的动力学参数预测生物降解的极限浓度，为后续的动态模拟和现场工程提供理论支持。
