Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Design, Tabriz Islamic Art University, Tabriz, Iran.
BMC Complement Med Ther. 2022 Nov 9;22(1):290. doi: 10.1186/s12906-022-03759-y.
The restorative effect of classical music was assessed on the cyclophosphamide-induced animal model of premature ovarian failure (POF).
Mozart's piano classical music (K.448) was used for up to 4 and 8 weeks. Rats were exposed to music 6 h every day using a stereo system with a volume of 65-70 dB. Sera and ovarian tissue samples were collected for the evaluation of FSH, LH, and E and histopathological examination. At the same time points, samples were taken from the hypothalamus and hippocampus to monitor the expression of Ntrk2, Crh, and Pomc using real-time PCR. Mating trial was performed to evaluate the fertility status of POF rats.
Histopathological examination revealed a significant increase (p < 0.05) in the numbers of morphologically normal follicles at all the developmental stages in POF rats after music therapy compared to the POF group (p < 0.05). Music therapy decreased FSH and LH levels to near-to-normal levels conidied with elevation of E (p < 0.05). Ntrk2, Crh, and Pomc expressions were down-regulated in POF rats. Music therapy increasaed the expression of Ntrk2 in the hypothalamus of POF rats (p < 0.05). In contrast, Crh and Pomc failed to reach the detection limit before intervention and four weeks after the intervention however, these genes were expressed eight weeks after music therapy. Fertility status was increased (p < 0.05) in terms of litter size in POF rats after being exposed to music compared to the non-treated POF control group (p < 0.05).
Results showed that music can exert therapeutic effects on POF rats via the alteration of sex-related hormones.
评估了古典音乐对环磷酰胺诱导的早发性卵巢功能衰竭（POF）动物模型的修复作用。
使用莫扎特的钢琴古典音乐（K.448）进行长达 4 至 8 周的治疗。使用音量为 65-70 dB 的立体声系统每天让老鼠暴露于音乐中 6 小时。收集血清和卵巢组织样本，以评估 FSH、LH 和 E，并进行组织病理学检查。在相同的时间点，从下丘脑和海马中取样，使用实时 PCR 监测 Ntrk2、Crh 和 Pomc 的表达。进行交配试验以评估 POF 大鼠的生育能力。
组织病理学检查显示，与 POF 组相比（p<0.05），音乐治疗后 POF 大鼠所有发育阶段的形态正常卵泡数量均显著增加（p<0.05）。音乐治疗可降低 FSH 和 LH 水平至接近正常水平，并升高 E（p<0.05）。POF 大鼠的 Ntrk2、Crh 和 Pomc 表达下调。音乐治疗可增加 POF 大鼠下丘脑的 Ntrk2 表达（p<0.05）。相反，Crh 和 Pomc 在干预前和干预 4 周后均未达到检测限，但在音乐治疗 8 周后这些基因被表达。与未经治疗的 POF 对照组相比（p<0.05），暴露于音乐的 POF 大鼠的生育能力（产仔数）增加。
结果表明，音乐可以通过改变与性别相关的激素对 POF 大鼠发挥治疗作用。
