Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Tsinghua-Berkeley Shenzhen Institute, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Biosensors (Basel). 2022 Nov 7;12(11):984. doi: 10.3390/bios12110984.
Rapid and cost-effective diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are a critical and valuable weapon for the coronavirus disease 2019 (COVID-19) pandemic response. SARS-CoV-2 invasion is primarily mediated by human angiotensin-converting enzyme 2 (hACE2). Recent developments in ACE2-based SARS-CoV-2 detection modalities accentuate the potential of this natural host-virus interaction for developing point-of-care (POC) COVID-19 diagnostic systems. Although research on harnessing ACE2 for SARS-CoV-2 detection is in its infancy, some interesting biosensing devices have been developed, showing the commercial viability of this intriguing new approach. The exquisite performance of the reported ACE2-based COVID-19 biosensors provides opportunities for researchers to develop rapid detection tools suitable for virus detection at points of entry, workplaces, or congregate scenarios in order to effectively implement pandemic control and management plans. However, to be considered as an emerging approach, the rationale for ACE2-based biosensing needs to be critically and comprehensively surveyed and discussed. Herein, we review the recent status of ACE2-based detection methods, the signal transduction principles in ACE2 biosensors and the development trend in the future. We discuss the challenges to development of ACE2-biosensors and delineate prospects for their use, along with recommended solutions and suggestions.
快速且经济有效的严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）诊断检测方法是应对 2019 年冠状病毒病（COVID-19）大流行的重要而有价值的手段。SARS-CoV-2 的入侵主要是通过人类血管紧张素转换酶 2（hACE2）介导的。基于 ACE2 的 SARS-CoV-2 检测模式的最新发展凸显了这种天然宿主-病毒相互作用在开发即时护理（POC）COVID-19 诊断系统方面的潜力。尽管利用 ACE2 进行 SARS-CoV-2 检测的研究还处于起步阶段，但已经开发出了一些有趣的生物传感器，显示了这种引人入胜的新方法的商业可行性。所报道的基于 ACE2 的 COVID-19 生物传感器的卓越性能为研究人员提供了机会，开发适合在入境点、工作场所或聚集场所进行病毒检测的快速检测工具，以便有效实施大流行控制和管理计划。然而，要被认为是一种新兴方法，基于 ACE2 的生物传感的基本原理需要进行批判性和全面的调查和讨论。本文综述了基于 ACE2 的检测方法的最新进展，ACE2 生物传感器中的信号转导原理以及未来的发展趋势。我们讨论了 ACE2 生物传感器发展所面临的挑战，并阐述了它们的应用前景，同时提出了建议和解决方案。
