Department of Earth, Environment and Life Sciences (DISTAV), University of Genoa, Corso Europa 26, 16132 Genoa, Italy.
Department of Chemical, Biological, Pharmaceutical and Enviromental Sciences (ChiBioFarAm), University of Messina, Viale Ferdinando Stagno d'Alcontres 31, 98166 Messina, Italy.
Toxins (Basel). 2022 Oct 24;14(11):726. doi: 10.3390/toxins14110726.
The misidentification between edible and poisonous plants is an increasing problem because of the new trend to collect wild plants, especially by amateur collectors who do not have the botanical skills to distinguish between edible and toxic species. Moreover, morphologically similar species are sometimes responsible for accidental contamination or used in the intentional adulteration of products for human and animal consumption. L. (laurel) and L. (cherry laurel) are typical ornamental shrubs of the Mediterranean region. Laurel is considered a non-toxic plant, widely used as flavorings. Conversely, cherry laurel leaves, morphologically similar to those of laurel, contain toxic cyanogenic glycosides. Considering this, the aim of this study was to carry out an in-depth evaluation of laurel and cherry laurel leaves by using light and scanning electron microscopy coupled with three step phytochemical analyses (qualitative and quantitative colorimetric assays and liquid chromatography). This allowed to highlight the distinguishing features of plant species investigated features such as the venation pattern, presence/absence of nectaries, calcium oxalate crystals, secretory idioblasts, and cyanogenic glycosides. Concluding, this multidisciplinary approach can be useful for the identification of plants but also fragments or pruning residues containing cyanogenic glycosides, in quality control tests, intoxications, and criminal cases.
由于采集野生植物的新趋势,尤其是那些不具备识别可食用和有毒物种的植物学技能的业余采集者,食用植物和有毒植物之间的错误识别问题日益严重。此外,形态相似的物种有时会导致意外污染,或者被用于故意掺假供人类和动物食用的产品。L.(月桂)和 L.(樱桃月桂)是地中海地区典型的观赏灌木。月桂被认为是一种无毒植物,广泛用作调味料。相反,樱桃月桂叶与月桂叶形态相似,含有有毒的氰苷。有鉴于此,本研究的目的是通过使用光和扫描电子显微镜结合三步植物化学分析(定性和定量比色分析和液相色谱)对月桂和樱桃月桂叶进行深入评估。这使得能够突出所研究植物物种的特征,例如叶脉模式、蜜腺的存在/不存在、草酸钙晶体、分泌型异形细胞和氰苷。总之,这种多学科方法可用于鉴定植物,也可用于质量控制测试、中毒和刑事案件中含氰苷的植物碎片或修剪残余物。