Lopes Lucas F, Bordin Keliani, de Lara Gabriel Hc, Saladino Federica, Quiles Juan M, Meca Giuseppe, Luciano Fernando B
School of Life Sciences, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Burjassot, Spain.
J Sci Food Agric. 2018 Jan;98(2):792-798. doi: 10.1002/jsfa.8527. Epub 2017 Aug 12.
Brazil produces approximately 40 000 tons of Brazil nuts annually, which is commonly contaminated with fungi and mycotoxins. Gaseous allyl isothiocyanate (AITC) was used to inhibit the growth of Aspergillus parasiticus and its production of aflatoxins (AFs) in Brazil nuts.
Nuts were inoculated with 10 spores g of A. parasiticus and placed in airtight glass jars with controlled relative humidity (RH = 95 or 85%). Samples were treated with 0, 0.5, 1.0 or 2.5 µL L of gaseous AITC and analyzed after 30 days to determine the fungal population and AFs content. Samples were also submitted to sensory evaluation. AITC at 2.5 µL L could completely inhibit the fungal growth and AFs production in both the RH tested. AITC at 0.5 and 1 µL L did not affect the microbial growth at RH = 95%, but 1 µL L reduced the production of AFs by ∼50%. All AITC treatments reduced the fungal population and AFs to undetectable levels at RH = 85%. None of the concentrations altered sensory characteristics of Brazil nuts.
Gaseous AITC could be used as an alternative to inhibit the growth of A. parasiticus during storage and transport of Brazil nuts. © 2017 Society of Chemical Industry.
巴西每年生产约40000吨巴西坚果，这些坚果通常受到真菌和霉菌毒素的污染。气态异硫氰酸烯丙酯（AITC）被用于抑制寄生曲霉在巴西坚果中的生长及其黄曲霉毒素（AFs）的产生。
将坚果接种10个寄生曲霉菌孢子/克，置于相对湿度可控（RH = 95%或85%）的气密玻璃罐中。样品用0、0.5、1.0或2.5微升/升的气态AITC处理，30天后进行分析以确定真菌数量和AFs含量。样品还进行了感官评价。在两种测试的相对湿度下，2.5微升/升的AITC均可完全抑制真菌生长和AFs产生。在RH = 95%时，0.5和1微升/升的AITC不影响微生物生长，但1微升/升可使AFs产量降低约50%。在RH = 85%时，所有AITC处理均将真菌数量和AFs降至检测不到的水平。没有任何浓度改变巴西坚果的感官特性。
气态AITC可作为一种替代方法，在巴西坚果的储存和运输过程中抑制寄生曲霉的生长。© 2017化学工业协会。
